A Comprehensive Study on Intrusion and Cooperation of Genomes

家蚕端粒相关非ltr反转录转座子TRAS1核酸内切酶结构域能够识别和切割端粒重复序列（TTAGG） n。本文以酵母PI-SceI为模型系统，研究了基因归巢的分子机制。分析了植物支原体突变株染色体外dna的基因组织、复制系统和表达及其与宿主特异性和致病性的关系，发现DEVDase的激活与环丁烷嘧啶二聚体相关，而uvc诱导的Medaka细胞凋亡与caspase-3样激活无关。通过分析体外合成的trna突变体的亮氨酸接受度，阐明了多聚密码子产生的分子机制。将HNI菌株的Medaka与T5测试菌株进行杂交，发现HNI菌株的雌性生殖细胞的自发总突变率明显高于雄性，但雌性生殖细胞的自发总突变率低于雄性，表明雌性生殖细胞的自发突变与较大的基因组缺失有关。从雌雄异株银叶（Silene latifolia）中分离出几个STS致病菌，并进行了单花粉分析和BAC无性系的分离。从进化的角度研究了光合机制对环境胁迫的抑制和驯化机制及其意义。利用稳定表达gfp -微管蛋白的活细胞，研究了高等植物细胞皮层微管的动态和作用。从海百合Metacrinus rotundus中克隆了调控基因Hox、Otx和engrailed，分析了其表达模式。从家蚕幼虫脑中分离出一种抑制外皮甾体生物合成的九残基肽。研究了嗅觉受体在功能重构系统中的配体特异性。肠隐窝细胞向上皮分化的特征是使用转染同源盒基因Cdx-2的IEC-6细胞。神经干细胞的增殖和分化受肿瘤抑制因子p53的调控。在新大陆灵长类动物和斑马鱼中发现了视觉色素基因的新成员及其基因组组织。少

Endonuclease domain of telomere associated non-LTR retrotransposon, TRAS1 of the silkworm could recognize and cleave the telomeric repeat (TTAGG) n. Yeast PI-SceI was used as a model system to study molecular mechanism of gene homing. Gene organization, replication system and expression of extrachromosomal DNAs from plant mycoplasma mutant lines and its relationship with host specificity and pathogenicity were analyzed, DEVDase activation was correlated with cyclobutane pyrimidine dimers, but the UVC-induced apoptosis occurred independent of the caspase-3-like activation in the Medaka cells. The molecular mechanism of emergence of polysemous codon was clarified by analyzing leucine acceptance of mutant tRNAs synthesized in vitro. Crossing of the Medaka of the HNI strain to those of the T5 tester strain revealed that the female germ cells of the HNI strain showed a remarkably higher spontaneous total mutation rates compared to that found for those of the males, however, the spontaneous … More viable mutation rates were lower in females than males, indicating that the spontaneous mutations in female germ cells are associated with larger deletions of the genome. Several STS makers were isolated from the dioecious plant, Silene latifolia, and then single-pollen analysis and isolation of a BAC clone were accomplished. Mechanism of inhibition and acclimation of photosynthetic machinery to environmental stress and its significance were studied from the evolutional point of view. Dynamics and roles of cortical microtubules in higher plant cells were investigated using living cells stably expressing GFP-tubulin proteins. The regulatory genes, Hox, Otx, and the engrailed were cloned from a sea lily, Metacrinus rotundus, for analyzing the expression pattern. A nine-residue peptide inhibiting ecdysteroid biosynthesis in the prothoracic glands was isolated from the larval brains of the silkworm, Bombyx mori. Ligand specificity of olfactory receptors was investigated in a functionally-reconstituted system. Differentiation of the intestinal crypt cells to the epithelium was characterized using IEC-6 cells transfected with a homeobox gene, Cdx-2. Proliferation and differentiation of neural stem cells are regulated by a tumor suppressor, p53. Novel members of visual pigment genes and their genomic organizations were revealed in New World primates and zebrafish. Less