Explanation of the Anticancer Mechanism and Clinical Chemotherapy for Hybrid Liposomes

杂化脂质体可以通过在缓冲溶液中简单地超声处理囊泡和胶束分子的混合物来制备。这些脂质体的物理性质，如大小、膜流动性、相变温度和疏水性可以通过改变组成来控制。在我们的研究过程中，杂种脂质体在体外无需药物的情况下对肿瘤生长具有非常高的抑制作用。杂交脂质体对体外正常细胞和体内正常大鼠均无毒性，无副作用。本文报道了由二myristoyl磷脂酰胆碱（DMPC）和聚氧乙烯烷基醚(C_<12>(EO)n；N =10-25)，在体外、体内及临床化疗中均对肿瘤细胞生长有抑制作用。本研究值得注意的方面总结如下：(a)得到了DMPC与C_<12>(EO)_n组成的杂化脂质体结构均匀稳定。(b)杂化脂质体对多种肿瘤细胞的体外生长具有高度抑制作用。(c)首次证实杂化脂质体通过激活死亡结构域和某些半胱天冬酶诱导细胞凋亡。(d)接种肿瘤细胞的小鼠经杂交脂质体处理后，存活时间明显延长，无任何副作用。(e)在临床应用中，对于淋巴瘤等肿瘤患者，经静脉注射杂交脂质体无需药物即可延长生存期，局部给予杂交脂质体可显著减少实体瘤。

Hybrid liposomes can be prepared by simply ultrasonicating a mixture of vesicular and micellar molecules in a buffer solution. The physical properties of these liposomes, such as size, membrane fluidity phase transition temperature, and hydrophobicity can be controlled by changing the composition. In the course of our study, remarkably high inhibitory effects of hybrid liposomes on tumor growth in vitro without drugs have been obtained. No toxicity of the hybrid liposomes was observed in normal cells in vitro and in normal rats in vivo without any side effect.In this study, we report on the uniform and stable hybrid liposomes composed of dimyristoylphosphatidylcholine (DMPC) and polyoxyethylenealkyl ether (C_<12>(EO)n; n=10-25) having inhibitory effects on the growth of tumor cells in vitro, in vivo and clinical chemotherapy.Asummary of the noteworthy aspects of this study is as follows : (a) the uniform and stable structure of hybrid liposomes composed of DMPC and C_<12>(EO)_n was obtained. (b) Highly inhibitory effects of hybrid liposomes were obtained on the growth of many kinds of tumor cells in vitro. (c) Induction of apoptosis by hybrid liposomes through activation of death domains and some kind of caspases was verified for the first time. (d) Significantly prolonged survival was obtained in mice inoculated tumor cells after the treatment with hybrid liposomes without any side effect. (e) In clinical application, the prolonged survival was attained in patient with lymphoma and other tumors after the intravenous injection of hybrid liposomes without drugs and the remarkable reduction of solid tumor was obtained after the local administration of hybrid liposomes.