Electrophysiological study of the mechanism of unconsciousness of anesthesia

方法：采用17-20日龄大鼠，观察抗麻醉药对麻醉药诱发的GABA能电流的影响。整个大脑被取出并在冰冷的盐水中切片。显微镜下鉴定丘脑LD细胞。全细胞记录和GABA能抑制电流记录，同时灌注伊恩溶液鼓泡与95%O2和5%CO2。首先记录麻醉剂（0.5%氟烷和0.5%七氟醚）的作用，然后将肉豆蔻酸酯或多沙普仑与麻醉剂一起应用10 ~ 15 min，并冲洗掉。

The effect of antianesthetic drug on the GABAergic current induced by anesthetics were investigated.Method : Rats (17-20 days old) were used. Whole brain was removed and sliced in the cold icy saline. Thalalmic LD cells were identified under microscope. Whole cell recordings were made and GABAergic inhibitory currents were recorded while perfused with Ian solution bubbled with 95% O2 and 5% CO2. The effect of anesthetics (0.5% halothane and 0.5% sevoflurane) was first recorded, and then myristate or doxapram was applied with anesthetics for 10 to 15 min. and washed out.Result : Anesthetic-induced augmentation of GABAergic currents was slightly subsided by myristate or doxapram.