Development of a tumor cell bank for next generation immunotherapy

本研究的目的是建立一种成功的、符合输血安全标准的肿瘤细胞系，并建立一种将这些肿瘤细胞用于治疗其他人的系统(用于治疗的肿瘤细胞库)。本研究期间获得的数据总结如下：1.用于治疗的肿瘤细胞系的建立：1)我们制备了96孔培养板，每孔含有不同浓度的自体血清或积液。2)利用微重力培养系统成功地对肿瘤组织中存在的肿瘤细胞进行长期培养。2.肿瘤细胞注册(肿瘤细胞库)：在6个肿瘤细胞系中，我们获得了5个患者作为肿瘤细胞库的注册许可，尽管我们从另外4个患者那里获得了注册许可，但没有成功地建立肿瘤细胞系。肿瘤细胞库的临床应用：在3例接受自体肿瘤冲击树突状细胞疫苗治疗的患者中，2例判断可以使用登记为肿瘤细胞库的非自身肿瘤细胞代替自身肿瘤细胞，1例耗尽自体肿瘤细胞的患者继续使用登记在肿瘤细胞库中的非自身肿瘤细胞进行疫苗治疗。4.肿瘤细胞库的公开性：我们展示了我们基于树突状细胞的疫苗治疗的临床数据，并在主页(http://www.tumor.med.kyushu-u.ac.jp/).5.)中介绍了肿瘤细胞库表明了使用注册到肿瘤细胞库的肿瘤细胞分泌的GMP级外切体进行无细胞疫苗治疗的可能性。

Purpose of this study is to develop a highly successful procedure for establishing tumor cell lines which conform to safety criteria of blood transfusion and to build a system to use these tumor cells for treatment of other humans (a tumor cell bank for treatments).Data obtained during this study period are summarized as follows :1.Establishment of tumor cell lines for treatment :1)We prepared a 96-well culture microplate in which each wells contain different concentrations of autologous sera or effusions. By using this microplate, 6 tumor cell lines were successfully established from 32 fresh carcinoma tissues.2)We succeeded in long-term culture of tumor cells existing in tumor tissue by using micro-gravity culture system.2.Registration of tumor cells for treatment (tumor cell bank) :Among the 6 tumor cell lines, we got registration permission from five patients as a tumor cell bank.Although we got registration permission from other 4 patients, we did not succeed in the establishment of a tumor cell line. These tumor tissues were frozen and stored as associate tumor cells for treatment.3.Clinical application of tumor cell bank :In 2 of 3 patients who are undergoing self tumor-pulsed dendritic cell-based vaccine therapy, it was judged to be able to use non-self tumor cells registered as tumor cell bank instead of self tumor cells.In one patient who used up self tumor cells, the vaccine therapy was continued by using non-self tumor cells registered to tumor cell bank. Through a treatment period, an antitumor immune response was maintained well, and no particular side effect was recognized.4.Publicity of tumor cell bank :We showed clinical data of our dendritic cell-based vaccine therapy and introduced tumor cell bank in a homepage (http://www.tumor.med.kyushu-u.ac.jp/).5. Possibility of cell-free vaccine therapy using GMP-grade exosomes secreted from tumor cells registered to tumor cell bank was indicated.