Course of SARS coronavirus-2-specific antibody titers and magnitude of the memory B-cell and T-cell response

大多数或所有SARS冠状病毒感染者都会对该病毒产生免疫反应。该研究的目的是检查轻度至中度SARS CoV-2感染个体的抗体滴度过程和病毒特异性记忆b细胞和t细胞反应的大小。50至60名感染SARS CoV-2的成年研究参与者将被随访12个月。感染后4至10周、6、9和12个月将获得血清样本。此外，在9和/或12个月时，将获得肝素化的血液标本以制备单个核细胞。血清将用于检测针对SARS CoV-2核蛋白和S糖蛋白受体结合域（RBD）、流感病毒核蛋白和破伤风类毒素的抗体反应以及中和抗体反应。细胞将用于测量SARS CoV-2核蛋白和RBD、流感病毒核蛋白、巨细胞病毒和破伤风类毒素特异性记忆B细胞和t细胞反应的大小。t细胞对RBD的反应将通过单个重叠肽更详细地观察。该研究测量了SARS cov -2特异性抗体在感染后的前六个月和六个月之后的反应过程，以及感染后9-12个月病毒特异性记忆B细胞和t细胞反应的强度。这些免疫反应将相互结合，以及对流感病毒、巨细胞病毒和破伤风抗原的免疫反应。该研究的一个特别重点是中和抗体反应与抗体、记忆B细胞和t细胞对病毒糖蛋白受体结合域的反应之间的关系。

Most or all SARS CoV-2-infected individuals develop an immune response to the virus. The goal of the study is to examine the course of the antibody titers and the magnitude of the virus-specific memory B-cell and T-cell responses in individuals with mild to moderate SARS CoV-2 infection.Fifty to sixty adult study participants with SARS CoV-2 infection will be followed up for 12 months. Four to ten weeks, 6, 9 and 12 months after infection serum samples will be obtained. In addition, at 9 and/or 12 months, heparinized blood specimens will be obtained to prepare mononuclear cells. Sera will be used to examine the antibody responses against the SARS CoV-2 nucleoprotein and the receptor binding domain (RBD) of the S glycoprotein, influenza virus nucleoprotein and tetanus toxoid as well as the neutralizing antibody response. Cells will be used to measure the magnitude of the SARS CoV-2 nucleoprotein and RBD, influenza virus nucleoprotein-, cytomegalovirus-, and tetanus toxoid-specific memory B- and T-cell response. The T-cell response against the RBD will be observed in greater detail with single overlapping peptides.The study measures the course of the SARS CoV-2-specific antibody response in the first six months and later than six months of infection and the magnitude of the virus-specific memory B- and T-cell response at 9-12 months after infection. The immune responses will be put into perspective to each other and to immune responses against influenza virus, cytomegalovirus and tetanus antigens. A special focus of the study is the association of the neutralizing antibody response with antibody, memory B- and T-cell responses against the receptor binding domain of the viral glycoprotein.