Light Sheet Fluorescence Microscope with Data Storage Module

光片荧光显微镜是一种非常光效率和快速的光学切片技术。当涉及到成像大的、透明的样品时，这是首选的技术。光片荧光显微镜的效率来自于激发和发射光路解耦的事实。这通过使用彼此垂直布置的两个物镜来实现：一个用于将照明光片投射到样品中，第二个用于检测来自激发的光学平面的荧光发射。我们申请了一种光片荧光显微镜，它可以以全自动的方式处理和成像非常大的标本。显微镜应通过高分辨率水浸检测物镜和数据存储模块进行扩展。光片显微镜将允许在体内成像以及透明和/或扩展的样品的成像。该系统包括四个用于荧光激发的激光器，范围从405至640 nm。该范围将提供宽范围的荧光团的有效激发，包括各种自身荧光蛋白，从而提供最大的标记灵活性。这种新仪器将用于开发样本扩展的新协议，并将应用于神经元连接组学，脑类器官和肾脏学领域的几个项目。

Light sheet fluorescence microscopy is an extremely light efficient and fast optical sectioning technique. It is the technique of choice when it comes to imaging large, cleared samples. The efficiency of light sheet fluorescence microscopy comes from the fact that the excitation and emission optical paths are decoupled. This is achieved by using two objective lenses arranged perpendicular to each other: One to project an illumination light sheet into the sample and the second one to detect fluorescence emission from the excited optical plane. We apply for a light sheet fluorescence microscope, which can handle and image very large specimen in a fully automated manner. The microscope shall be extended by a high resolution water-dipping detection objective and a data storage module. The light sheet microscope will allow in vivo imaging as well as the imaging of cleared and/or expanded samples. The system includes four lasers for fluorescence excitation ranging from 405 to 640 nm. This range will provide the efficient excitation of a broad range of fluorophores including various autofluorescent proteins giving maximal flexibility of labeling. This new instrument will be used to develop new protocols for specimen expansion and will be applied to several projects in the fields of, neuronal connectomics, brain organoids and nephrology.