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MRI: Acquisition of Multimodal Light Sheet Fluorescence Microscope Workstation to Accelerate Multi-Disciplinary Research and Impact STEM Outreach in Northern Indiana

MRI：收购多模态光片荧光显微镜工作站，以加速印第安纳州北部的多学科研究并影响 STEM 推广

基本信息

批准号：
1919832
负责人：
Jeremiah Zartman
金额：
$ 46.88万
依托单位：
University of Notre Dame
依托单位国家：
美国
项目类别：
Standard Grant
财政年份：
2019
资助国家：
美国
起止时间：
2019-09-15 至 2022-08-31
项目状态：
已结题

来源：
https://www.nsf.gov/awardsearch/showAward?AWD_ID=1919832&HistoricalAwards=false
关键词：
MRI Acquisition Multimodal Light Sheet

项目摘要

This Major Research Instrumentation award supports the acquisition of a light sheet fluorescence microscope workstation system at the University of Notre Dame in Indiana. The light sheet fluorescence microscope system will be available as a shared instrument managed by the Notre Dame Integrated Imaging Facility and its expert, permanent staff. Given that the facility is connected to a shared network, it also will be accessible to many institutions throughout Indiana. Access to the light sheet fluorescence microscope will provide cutting-edge training opportunities for a diverse range of undergraduate, graduate, and post-doctoral researchers. The faculty and their trainees, supported by the Notre Dame Integrated Imaging Facility staff, will undertake a range of educational and outreach activities using the light sheet fluorescence microscope workstation. The light sheet fluorescence microscope workstation system will expand and amplify the University of Notre Dame's contributions in advancing innovative engineering and science research, education and community outreach.This microscope enables three-dimensional images to be acquired at unprecedented speeds and improved axial resolution compared to traditional confocal microscopes. In addition to fast speeds and larger samples, three-dimensional stem-cell derived organoids or small model organisms (e.g., zebrafish, fruit flies, or frog embryos) can be imaged without damaging samples with the illumination light. The reduced bleaching and phototoxicity enables long-term imaging over many days. This extended timeframe and additional photomanipulation modalities dramatically expand the range of possible experiments that can be conducted. The multimodal microscope enables unprecedented capabilities for investigating the dynamics of cell behavior in a broad range of three-dimensional samples, including epithelia, brain, eyes, heart, and kidney tissues. The tool will allow new questions to be answered on how normal and genetically modified cells interact at the systems level. The tool also will facilitate new analyses of engineered vascularized tissue models and biorobots by enabling both photomanipulation and fast imaging.This award reflects NSF's statutory mission and has been deemed worthy of support through evaluation using the Foundation's intellectual merit and broader impacts review criteria.

这一重大研究仪器奖支持印第安纳州圣母大学收购光片荧光显微镜工作站系统。光片荧光显微镜系统将作为一个共享仪器提供，由巴黎圣母院综合成像设施及其专家、常设工作人员管理。鉴于该设施连接到一个共享网络，印第安纳州的许多机构也将可以访问它。使用光片荧光显微镜将为不同范围的本科生、研究生和博士后研究人员提供尖端培训机会。教员及其受训人员将在巴黎圣母院综合成像设施工作人员的支持下，利用光片荧光显微镜工作站开展一系列教育和外展活动。光片荧光显微镜工作站系统将扩大和放大圣母大学在推进创新工程和科学研究、教育和社区外展方面的贡献。与传统的共焦显微镜相比，这种显微镜能够以前所未有的速度获取三维图像，并提高轴向分辨率。除了更快的速度和更大的样本，三维干细胞衍生的有机物或小型模型生物(例如斑马鱼、果蝇或青蛙胚胎)可以在不破坏样本的情况下进行照明成像。减少了漂白和光毒性，使长期成像可以持续很多天。这一延长的时间框架和额外的光操纵模式极大地扩大了可以进行的可能实验的范围。多模式显微镜能够在广泛的三维样本中研究细胞行为的动力学，包括上皮、脑、眼睛、心脏和肾脏组织，这是前所未有的能力。该工具将允许回答有关正常细胞和转基因细胞如何在系统层面上相互作用的新问题。该工具还将通过实现光操纵和快速成像，促进对工程化血管组织模型和生物机器人的新分析。该奖项反映了NSF的法定使命，并通过使用基金会的智力优势和更广泛的影响审查标准进行评估，被认为值得支持。