Development of a rapid, Mgh sensitive ard simple method for the disrupters in environment

一种基于表面等离子体共振（SPR）的简单分析仪器，用于环境样品中快速筛选毒性有机物质的野外使用。自从第一次，常规匹配油以来，作为棱镜和涂层玻璃之间的光界面使用，是不适合野外使用的，我们开发了一种基于塑料PVC的新的光界面薄膜。薄膜性能最佳在0.5二-乙基己烷至0.5克聚氯乙烯在0.2克聚氯乙烯中的比率为80-CSCond，以提高SPR信号的敏感性，使抗IgG到Au表面的有效不流动性。较大的SPR信号在IgG和抗IgG反应之间使用10-碳基1-十二烷醇作为一种治疗剂在测试中与三种不同的剂量，从广义上讲，良好的校准曲线在0.6 - 3.O × 10-3 mM 2，4-二氯酚中发现了类似于二恶英的治疗剂。它很清楚，因为SPR化学传感器可以被构建为具有毒性低分子有机物质的环境荷尔蒙，如环境荷尔蒙，由开发的更有效的抗移动性技术--Au表面上的抗体技术。

A simple analytical instrument based on surface plasmon resonance(SPR) for the the rapid screening toxic organic substance in environment samples has been needed for field use.First, since conventional matching oil, which is used as the light interface between a prism and an Au coated cover glass, is unsuitable for field use, we developed a new light interface membrane based on a plasticized PVC. The membrane performance was best in the ratio of 0.5 di-2-ethylhexyl phthalate to 0.5 g tritol phosphate in 0.2g PVC at 80ーCSecond, in order to enhance the sensitivity of SPR signal, the efficient immobilization of anti-IgG to Au surface was investigated. The large SPR signal was obtained in the immuno-reaction between IgG and anti-IgG using 10-Carboxyl 1-decanethiol as the treatment agent in the test with some kinds of thiols.Consequently, the good calibration curve was obtained in the range 0.6〜3.O× 10-3 mM 2, 4-di-chlorophenol, which was similar to dioxin.From these experimental results, it became clear that SPR chemical sensor for the toxic low molecule organic substance like environment hormones could be constructed by development of the much more efficient immobilization-technique of antibody on Au surface.