Development of Novel Separation Media Using Photo-functioning Host Compounds

在新功能化学物质的发明、生物化学或生物学机理的阐明或环境污染物的寻找等方面，柱层析、薄层层析或液相层析等是必不可少的分离方法，在这些分离方法中，为了调节分离特性，经常使用有机溶剂作为萃取剂，或移动的添加剂。有许多对生态系统有害的溶剂。本研究提出了一种改变固定相特性而不是改变移动的相特性的分离技术。本实验以γ-环糊精（CD）作为光敏分子识别位点，制备了键合在硅胶上的AzCD硅胶填料。采用反相微液相色谱法考察了其分离特性及对紫外和可见光的响应性。以7种多环芳烃为模型样品。当用紫外光照射该填料时，较早洗脱的样品分子（例如并五苯）的保留时间显示出相对较小的保留减少，而具有宽峰宽度的分子（例如二萘嵌苯等）的保留时间显示出显著增加的保留。

In inventions of new functional chemical substance, elucidation of the biochemical or biological mechanisms or search for the environmental pollutants, etc., it is a column, thin layer or liquid chromatography, which are indispensable separation methods.In these separation methods, organic solvent is often used as extractant, or mobile phase additives in order to adjust the separation characteristics. There are many solvents of being noxious for the ecosystem. In this study, separation technique which could changes the characteristics of the stationary phases instead of the characteristics of mobile phase was demonstrated. It is safe because changing the property of stationary phase itself by external field (light), and that it is also gentle for the environment.In our typical case, γ- cyclodextrin (CD) was made to be the photoresponsivity molecular recognition position, and the packings (the AzCD silica ) which was bonded in the silica gel was prepared. The separation characteristics and the responsibility for ultraviolet and visible light were examined using reversed-phase micro liquid chromatography. Seven kinds of polycyclic aromatic hydrocarbons were used as model samples. When this packing was irradiated with the ultraviolet light, the retention times of the sample molecules eluting earlier, such as the pentacene, showed comparatively small decrease of retention, and the retention time of the molecule with wide peak width, such as perylene, etc. showed reversely increased retention.