GC-atmospheric pressure laser ionisation-HR-time-of-flight mass spectrometer with FF-TG-GC option

利用现有的气相色谱-大气压激光电离-高分辨率飞行时间质谱仪(GC-APLI-TOFMS)和高效液相色谱(LC)选项(2010年资助)，建立了多环芳烃(PAH，环境污染物)的分析方法，与普通的GC-MS PAH分析方法相比，其灵敏度提高了约1000倍。据我们所知，多环芳烃的检测下限尚未公布。由于成功的方法开发，以及由于LC的额外替代使用，APLI-MS系统现在已被用于不同应用的满负荷。用GC-或LC-APLI-TOFMS分析大气颗粒物(PM)、烟尘、土壤、沉积物、成岩基质、室内空气、地表水、海水、地下水等中的多环芳烃，并测定尿中多环芳烃的代谢物。在合作中，还对选定的PM和沉积物中的毒性效应进行了额外研究。此外，在最古老的前寒武纪岩石中首次检测到芳香类固醇，这在以前是不可能的。其他应用领域是地表水和河岸过滤中的农药和药物(靶标、非靶标和协同效应导向分析)，以及用于确定类黑碳结构的苯多羧酸分析。特别是，GC-APLI-TOFMS方法由于其选择性，代表了多环芳烃分析的一个里程碑，并可能在未来通过开发一种经济高效的紧凑系统来取代普通的多环芳烃分析。在这里，作为第一步，将研究流场-温度梯度-GC(FF-TG-GC)加速层析的适用性以及所用KRF准分子激光器与DPSS激光器的可比性。作为对仪器硬件的二次开发，我们计划建立一个高效导向的GC-APLI-TOFMS-循环LC和微量EROD分析方法。通过这种方式，选择性地获得了关于多环芳烃和毒性效应的平行信息。有了现有的APLI-MS系统，不再可能在硬件上进行全面的工作来开发新的方法，因此，这里应用了第二个相同的GC-APLI-TOFMS系统，并增加了一个FF-TG-GC选项。

By the available gas chromatography-atmospheric pressure laser ionization – high resolution time-of-flight mass spectrometer (GC-APLI-TOFMS) with high performance liquid chromatography (LC) option (funded in 2010), methods for the analysis of polycyclic aromatic hydrocarbons (PAH, environmental pollutants) were developed which are more sensitive by a factor of about 1,000 compared to common GC-MS PAH analysis. To our knowledge, lower limits of detection for PAH have not yet been published. As a result of the successful method developments and due to the additional alternative use with LC, the APLI-MS system is now used to full capacity for the different applications. PAH are analyzed by GC- or LC-APLI-TOFMS in atmospheric particulate matter (PM), soots, soils, sediments, technogenic substrates, indoor air, surface water, sea water, ground water, etc. and PAH metabolites are measured in urine. In cooperation, toxic effects were additionally studied in selected PM and sediments. Furthermore, aromatic steroids were detected for the first time in oldest precambrian rocks, where this had not been possible before. Other application fields are pesticides and pharmaceuticals (target, non-target and in cooperation effect-directed analysis) in surface water and river bank filtration as well as benzenepolycarboxylic acid analysis for the determination of black carbon-like structures. Particularly, the GC-APLI-TOFMS method represents a milestone in PAH analysis due to its selectivity and may replace common PAH analysis by development of a cost-efficient compact system in the future. Here, as a first step, the suitability of an accelerated chromatography by flowfield-temperature gradient-GC (FF-TG-GC) and the comparability of the used KrF eximer laser with a DPSS laser will be studied. As a second method development with comprehensive work on the hardware of the instrument, an effect-directed GC-APLI-TOFMS with recycling-LC and mini EROD-assay is planned. By this, parallel information selectively about PAH and toxic effects are gained. With the available APLI-MS system, new method developments with comprehensive work on hardware is not possible anymore, therefore, a second identical GC-APLI-TOFMS system with an additional FF-TG-GC option is applied for here.