The Evaluetion of The Hypersensitivity Depression Effect Using The Hypersensitive Dentine Simulator

根据流体力学理论，牙本质感知是由牙本质微管内部流体的转移引起的，因此，测量牙本质微管内部流体转移的阻吓性的技术似乎有助于客观评价超敏的牙本质控制物质。本研究使用10颗拔除的人类第三磨牙。在牙轴垂直表面制备牙冠部分1和1.5mm厚度的牙本质切片。这些切片固定在10%中性福尔马林溶液中。实验中打开牙本质微管，用30%过氧化氢水清洗。从牙冠牙本质微管开口的两侧和牙髓侧，用透明丙烯酸管将牙本质切片用o形环固定。在牙髓侧的丙烯酸管中加入1%氢氧化钠溶液，用0.02%鲁米诺填充。采用Millipore公司孔径为0.2 μm的过滤器对牙冠侧丙烯酸管中的细小颗粒进行了同样的去除，效果满意。光电二极管（s6204: Hamamatsu Photonics Co.）包含前置放大器和热电冷却装置，在样品附近有大约30mm的距离。装有样品的设备安装在几乎完全隔绝外界光线的黑盒子里。过氧化氢水的活性氧引起的鲁米诺化学发光的发光是由填充在两侧丙烯酸管中的试剂的混合物通过牙本质微管产生的。在光电二极管中检测到发光现象。光电二极管的光输出经示波器观察后，通过AD转换器记录在个人计算机中。在试剂仅与牙本质切片两侧接触的情况下，有轻微的瞬态发光。对填充了铁氰化钾和过氧化氢溶液的丙烯酸管施加10 ~ 500mmHg的气压，观察到光发射随时间的变化。这些发光量与同一样品的压力呈正相关，但在单个样品中存在比例波动，发光量发生变化。在使用1.0mm厚度的片状样品时，其牙本质的透光性较好，在发射量上，其发射量总体上比1.5mm的样品有增加的趋势。在30%过氧化氢水不打开牙本质微管的情况下，即使在任何负载的试剂中，牙本质样品也不能显示出发光。牙本质超敏模拟器是一种利用化学发光技术将牙本质微管内部流体的转移可视化的系统，它可以超敏化，并且可以被观察到。在发光量波动的情况下，可以显示牙本质微管的开放程度。该系统作为一种能够直观评价牙本质过敏症对照材料效果的工具，具有很大的实用价值。少

Dentine perception is evoked by the transfer of internal fluid in the dentine microtubule according to the hydrodynamics theory, therefore, the technique which measures the deterrence of the transfer of dentinal tubule internal fluid seems to be useful in order to objectively evaluate the hypersensitive dentine control material. Ten extracted human third molar teeth were used in this study. The dentine slices at the crown part of 1 and 1.5mm thickness were prepared in tooth axis vertical surface. These slices were fixed in the 10% neutral formalin solution. The dentine microtubule were opened to wash in the experiment by 3O% hydrogen peroxide water. From both slice sides where the dentine microtubule in the tooth crown and tooth pulp side opened, these dentine slices were held with the O-ring by the transparent acrylic tube. 1% sodium hydroxide solution in the acrylic tube in the tooth pulp side with the 0.02% luminol was filled. 1% potassium ferricyanide solution including the 0.3% hy … More drogen peroxide water which similarly removed the fine particle in the acrylic tube in the tooth crown side by the Millipore Corp. filter of ostia size of 0.2 μm was satisfied. The photodiode (s6204 : Hamamatsu Photonics Co.)which contained preamplifier and thermoelectric cooling device by having approximately 30mm distances in the neighborhood of the sample was installed. The equipment with sample was installed in the black box which almost perfectly cuts off the outside light. The light emission of luminol chemiluminescence evoked by the active oxygen of hydrogen peroxide water is generated by the mixture of the reagents filled in the acrylic tube of both sides through dentine microtubules. The luminous phenomenon was detected in the photodiode. The optical output from the photodiode was recorded through the AD converter in personal computer after observing by the oscilloscope. In the condition that the reagent just contacted both dentine slice sides, there was a slight light emission of the transience. The light emission was observed time-dependent, when 10〜500mmHg air pressure was applied for the acrylic tube that the potassium ferricyanide and hydrogen peroxide solution was filled. These light emission quantity had the correlation which was positive with the pressure in the same sample, though there is the fluctuation in proportion to individual sample, and the light emission quantity changed. At the light emission quantity in using slice samples of the 1.0mm thickness which was excellent in the permeability in the dentine, the light emission quantity tended to generally increase in comparison with 1.5mm samples. In the condition without opening of dentine microtubule by 30% hydrogen peroxide water, the dentine sample could not demonstrate the light emission even in any loading of reagent. It is a system that dentine hypersentive simulator using the chemiluminescence visualizes the transfer of the internal fluid in dentine microtubule and that it can be supersensitized, and that it can be observed. It is possible to show the degree of the opening of the dentine microtubule in fluctuation situation of the light emission quantity. This system seems to be very much the usefulness as the tool which can straightforwardly evaluate the effect of the control material for dentine hypersensitive disease. Less