Studies about lung metastasis and regional lymph node metastasis in SCC

高恶性鳞状细胞癌（SCC）中，FI细胞系有发生肺转移、局部淋巴结转移的潜力；裸鼠和患者的淋巴结外扩散、高钙血症、白细胞增多和恶病质。一只携带FI肿瘤的小鼠由于其恶性肿瘤发生恶病质并在一个月内死亡。将来自肺转移的细胞系和来自区域淋巴结转移的细胞重复接种于裸鼠皮下，我们分别建立了易于转移到肺和区域淋巴结的L细胞和N细胞。与亲本FI细胞的特点相比，形态学上，L细胞和N细胞的细胞间接触相对松散。L细胞（体积：1 × 10^5/cm^3）比FI细胞（5 × 10^4/cm^3）发生更严重的白细胞增生。L细胞条件培养基（CM）中G-CSF （20 ng/ml, 48h）的产生量约为FI细胞的50倍以上。N细胞CM中G-CSF的产量（1 ng/ml, 48h）比FI细胞高约10倍。由于FI细胞有G-CSF受体（G-CSF Receptor, G-CSFR）表达，使用rG-CSF和抗G-CSF中和抗体进行FI细胞和L细胞增殖相关实验，提示G-CSF可能是FI细胞的弱自分泌生长因子。接下来，在口腔鳞状细胞癌患者中，我们研究了G-CSF和G-CSFR的表达是否与肺转移或淋巴结转移有关。结果如下：(1)106例口服SCCs中，56例（53%）G-CSFR阳性，56例（53%）G-CSF阳性。(2) G-CSFR表达与肿瘤大小（P=0.0078）、疾病分期（P= 0.0084）、死亡（P=0.012）、G-CSF表达（P=0.05）相关；(3)G-CSF表达与肿瘤大小（P=0.039）、G-CSF表达（P=0.05）相关。(4)与区域淋巴结转移和肺转移均无相关性。g - csfr阳性患者的5年生存率为78%，g - csfr阴性患者的5年生存率为46%，差异有统计学意义。上述结果提示G-CSFR的表达与口腔鳞状细胞癌患者的预后相关。少

A high malignant squamous cell carcinoma(SCC), FI cell line has a potential to develop lung metastasis, regional lymph nodes metastasis ; extra-nodal spreads, hypercalcemia, leukocytosis and cachexia in nude mice as well as in the patient. A mouse bearing FI tumor developed cachexia and died within a month, because of its malignancy. Repeating several procedures which the cell line derived from lung metastases, the other cell from regional lymph nodes metastases are again inoculated subcutaneously to nude mice, we established L cell and N cell which are easy to develop metastases to lung and regional lymph nodes, respectively. Compared with characteristics of parental FI cell, morphologically, L cell and N cell have relatively loose cell-cell contact. L cell (W.B.C./Volume : 1 X 10^5/cm^3) developed leukocytosis more severe than that of FI cell (5 X 10^4/cm^3). Granulocyte-Colony Stimulating Factor (G-CSF) production (20 ng/ml for 48h) in the conditioned media(CM) of L cell were about … More 50 times higher than those of FI cell. G-CSF production (1 ng/ml for 48h) in the CM of N cell were about 10 times higher than that of FI cell. As FI cell has G-CSF Receptor (G-CSFR) expression, the experiments related with FI cell and L cell proliferation used rG-CSF and anti-G-CSF neutralizing antibody, indicated that G-CSF might be weak autocrine growth factor for FI cell. Next, in patients with oral SCC we investigated whether G-CSF and G-CSFR expression were generally related with lung metastases or lymph nodes metastases or not. The results were as follows (1) Out of 106 oral SCCs, 56 cases(53 %) were positive for G-CSFR and 56 (53 %) were positive for G-CSF. (2) Expression of G-CSFR correlated significantly with Tumor size (P=0.0078), Stage of disease(P=0,0084), Death(P=0.012) and Expression of G-CSF(P=0.05), (3) Expression of G-CSF correlated significantly with Tumor size(P=0.039) and Expression of G-CSF(P=0.05). (4) None of them correlated with regional lymph nodes metastases nor lung metastases. 5-year-survival-rate was 78 % in G-CSFR-positive, 46 % in G-CSFR-negative, respectively and these are statistically significant.These results indicated that expression of G-CSFR correlates with prognosis of patients with oral SCC. Less