Development of Recombined Plants Enzyme Genes for Product Useful Compound

研究了外源合成酶基因对次生代谢物产生的影响1)介绍了莱氏杜氏藻和白色梭子蟹。Duboisia和Hyoscyamus H6H-基因将Hyoscyamine代谢为东莨菪碱。共诱导了12株杜氏真核细胞株和8株含H6H基因的白骨壤细胞株。讨论了它们的能力，产物东莨菪碱。在杜波依斯的一些细胞系中发现了两种新的未知生物碱；2)在杜波依斯的18个细胞系和贝塔的13个细胞系中发现了能将阿魏酸代谢成香草醛的雷公藤和甜菜的HCHL基因(2)。然而，他们的基因在前者中并未被揭示。在后者中，茉莉酸甲酯诱导的m-RNA和4-羟基苯甲酸和香草酸的产物存在，但不存在香草素。3)尖叶茄子：该植物通过乙酸-罗巴酸途径产生葡萄糖苷。HMGR是受HMGR-激酶调控的途径中的关键酶。将HMGR-Kinase(NPK5)基因(3)、NPK5-K48R基因(4)和糖苷酶基因(5)导入该植物。但含有这些基因的细胞系(3，4)没有得到。基因修饰的糖苷酶基因(5)抑制了甾体皂苷的生成。

An Influence of external genes which synthesis enzyme, on Second metabolic substance productions were studied.1)Duboisia leichhardtii and Hyoscyamus albus : We introduced to. Duboisia and Hyoscyamus H6H-gene which metabolize hyoscyamine to scopolamine. 12 cell lines of Duboisia and 8 cell lines of Hyoscyamus which had H6H gene were induced. Their ability, of product scopolamine were discussed. Some cell lines in Duboisia indicated higher scopolamine product and Some cell lines in Hyoscyamu indicated higher hyoscyamine product : Two new unknown alkaloids were found In the latter some cell lines.2)D. leichhardtii and Beta vulgaris HCHL gene(2) which metabolize ferulic acid to vanillin existed in 18 cell lines of Duboisia and 13 cell lines of Beta. However their genes were not revealed in the former. In the latter, m-RNA induced from their genes existed by methyl jasmonate treatment, and 4-hydroxybenzoic acid and vanillic acid product but did not vanillin.3)Solanum aculeatissimum : this plant product glygoside via pathway acetic acid-rnebaronic acid. HMGR is key enzyme in the pathway was controled by HMGR-kinase. HMGR-kinase (NPK5) gene (3)NPK5-K48R gene (4)which was modified NPK5 and glycoside enzyme gene(5) were introduced to this plant. But cell line contained these genes(3,4) did not gotten. The, gene modified glycoside enzyme gene(5) inhibited steroidal saponin product.