BREEDING OF HIGH DIGESTIBLE RICE PLANT FOR FEED USING SOMACLONAL VARIATION.

为了利用组织培养技术培育出优质的反刍动物饲料用水稻植株，本研究以成熟水稻种子(cv.Nihonbaar)。在初始培养28d时，形成了被致密愈伤组织覆盖的胚性结构。继代培养通过体细胞胚胎发生在再分化培养基上再生出89株植株，随机选择53个再生计划，移栽到1/5000A的盆栽中，然后转移到室外。37个对照植株在相同的条件下生长，这些对照植株是从日本大麦的个体种子中获得的。再生植株有晚出穗的趋势。此外，与对照相比，它们具有更多的穗数，更少的种子重量和木质素含量，更高的叶茎比。在植株长度、干物质含量(…)方面没有观察到显著差异。再生剂和对照的产量、干物质消化率和可消化干物质产量均高于对照。在大多数测定中，再生剂的取值范围比对照宽，两组间的分布规律也不同。再生体组的出穗时间明显长于对照组。3个品系中有一个品系的可消化干物质产量高于对照组。再生剂组的可消化干物质产量变化较大。种子在再生剂中的重量与对照相当或更小。在大多数测量中，再生剂的取值范围比对照更宽，且两组之间的分布规律不同。人们认识到，再生体组中的一些植株种子较多，叶和茎中的可消化干物质较多，尽管种子较少，但可消化干物质显著。我们在再生植株中获得了比对照“日本稻”更高的可消化植株，但由于性状尚未确定，需要进行后续试验。较少

In order to produce the high quality rice plant for ruminant feed by the tissue culture technique, we carried out the experiments as follows ;The calli were induced from the scutellum of matured rice seed (cv. Nihonbare). At 28 days after initial cultivation, the embryogenic structures covered with the compact callus was formed. The successive cultivation regenerated the 89 plants from the re-differentiation medium through the formation of somatic embryogenesis.Fifty-three regenerated plans were randomly selected, transplanted into pots (1/5000a) and transferred to the outdoors. Thirty-seven control plants, which were obtained from the individual seed of Nihonbare, were grown under the same conditions. The regenerated plants have a tendency to be late for the ear emergence. Additionally, they have more number of ear, less seeds weight and lignin content and higher leaf/stem ratio, compared with the control. It was not observed the significant differences in the plant length, the dry ma … More tter yield, the dry matter digestibility and the digestible dry matter yield between the regenerants and the control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups.Successively, the promising 3 lines were selected from the regenerated plants and were grown under the same condition above. The term of ear emergence of in the regenerant group is longer than the control groups. One of 3 lines produced more the digestible dry matter than the control groups. The digestible dry matter yields were more variable in the regenerant group. The weights of seed in the regenerants were equivalent or less compared with control. In the most of measurements, the range of values is wider in the regenerant than in the control and the distribution pattern is different between two groups. It was recognized that some plants in the regenerant group have the much seed and digestible dry matter in leaf and stem, and have the remarkable digestible dry matter though having less seed. We obtained the higher digestible rice plants in the regenerant plants than in the control "Nihonbare", but are required the successive experiment because the trait is not fixed yet. Less