Research On MALT Lymphoma As a Model of Multistep Carcinogenesis

研究了G-I道、眼附件和甲状腺MALT淋巴瘤中粘附分子的表达。α4s7整合素和l -选择素在低级别MALT淋巴瘤细胞和高级别MALT淋巴瘤低级别部分检测到，而在高级别MALT淋巴瘤高级别部分和弥漫性大b细胞淋巴瘤中未检测到。大多数眼附件MALT淋巴瘤仅l -选择素阳性。在甲状腺中均未检测到。α4s7整合素的配体MAdCAM-1在HEV的g - i道和甲状腺的炎性病变和MALT淋巴瘤组织中表达，但在眼附件和涎腺中不表达。2.t(11;18)（API2/MALT1）在MALT淋巴瘤中的表达采用FISH和RT-PCR进行研究。API2/MALT1易位在一小部分眼附件和大肠MALT淋巴瘤中发现，在弥漫性大b细胞淋巴瘤和反应性淋巴网状增生中也很少发现，但在唾液腺和甲状腺MALT淋巴瘤中未发现。根除幽门螺杆菌可使88%的胃MALT淋巴瘤患者完全缓解，但对PI2/ malt1阳性患者无效。对包括MALT淋巴瘤在内的眼附件淋巴细胞增生性疾病的临床病理和免疫遗传学特征进行了分析。本文分析了多器官MALT淋巴瘤的临床病理特征。MALT淋巴瘤患者外周血单个核细胞在细胞因子刺激或HSP60联合刺激下CD40配体表达增加，IL4产生增加。DNA微阵列分析显示HLA-DR和整合素mrna水平升高。

1Eppression of adhesion molecules was investigated in MALT lymphomas of the G-I tract, ocular adnexa, and thyroid. α4s7 integrin and L-selectin were detected on low-grade MALT lymphoma cells and low-grade portions of high-grade MALT lymphoma, but not on high-grade portions of high-grade MALT lymphoma or diffuse large B-cell lymphomas. Most MALT lymphoma cases of the ocular adnexa were positive only for L-selectin. Neither was detected in the thyroid. MAdCAM-1, a ligand for α4s7 integrin, was expressed on the HEV in the inflammatory lesions and MALT lymphoma tissues of theG-I tract and thyroid, but not in the ocular adnexa or salivary gland.2.t(11;18)(API2/MALT1) in MALT lymphomas was investigated using FISH and RT-PCR. API2/MALT1 translocation was detected in a small portion of ocular adnexal and large intestinal MALT lymphomas and also rarely in diffuse large B-cell lymphomas and reactive lymphoreticular hyperplasia, but not in MALT lymphomas of the salivary gland and thyroid.3.Eradication of Helicobacter pylori induced complete remission in 88% of the patients with gastric MALT lymphoma, but not effective in the PI2/MALT1-positive cases.4.Clinicopathological and immunogenetic features of ocular adnexal lymphoproliferative disorders including MALT lymphomas were characterized.5. Clinicopathological features of multiple organ MALT lymphomas were characterized.6. Peripheral blood mononuclear cells in patients with MALT lymphoma showed increased expression of CD40 ligand and augmented production of IL4 by stimulation with cytokine or combined stimulation with HSP60. DNA microarray analysis indicated increased levels of HLA-DR and integrin mRNAs.