High-throughput three-dimensional micro imaging using WDM technolgies

本研究旨在融合光波波长域复用与二维空域并行的思想，实现高带宽的三维显微成像。为了实现高通量系统，我们需要各种新的子系统技术，这些子系统技术涉及二维平行光学器件、衍射光学元件、集成电路等的基本特性。特别是，并行结构，这是必不可少的，以实现高吞吐量，提出了新的要求，光学系统的设计。例如，在传统的显微镜物镜中，由于不应该进行离轴光束探测的透镜系统的严重畸变，来自激光器的离轴光束不能成像到适当的成像位置上。显然，一个系统级的问题发生，由于引入到成像application.To解决这样的问题，我们开发了主动对准的目的，并行系统的稳定性，并行共焦成像的最佳透镜设计，并通过使用计算传感器阵列，这是直接适用于并行成像光学系统的数据处理。实验证明，在118 Hz的环境干扰，成功地减少了主动对准。同时，利用H ∞控制理论，实现了控制器动态特性与干扰的综合，实现了主动对准。对于平行共焦成像系统，通过减少畸变和像差来开发，如果我们假设共焦显微镜的间距为250 μ m，则其支持多达3，200个平行通道。

This research intends to achieve high-bandwidth three-dimensional micro imaging by fusing the idea of wavelength-domain multiplexing of light-wave and two-dimensional space-domain parallelism. For realize high-throughput systems, we need various new sub-system technologies concerning the essential properties of two-dimensional parallel optical devices, diffractive optical elements, integrated circuits, and so forth. In particular, the parallel structure, which is indispensable to achieve high-throughput, imposes new requirements to optical system designs. For example, in a conventional microscope objective, off-axis beams from a laser cannot be imaged onto a proper imaging position due to severe distortion of the lens system where the off-axis beam probing is not supposed. Clearly, a system-level problem occurs due to the introduction of parallelism into imaging applications.To solve such problems, we developed active alignment for the purpose of parallel system stabilization, optimal lens design for parallel confocal imaging, and data processing by using computational sensor array which is directly adapted to a parallel imaging optics. The active alignment was experimentally demonstrated where 118 Hz environmental disturbance was successfully reduced. Also, by employing H-infinity theory, we also realized to integrate the dynamics of the controller and disturbance for the operation of the active alignment. For the parallel confocal imaging system was developed by reducing distortion and astigmatic aberrations, which supports up to 3,200 parallel channels if we assume 250um pitch for the confocal microscopy.