Development Of Chemiluminometric μ-Flow-Through Detectors

设计了一种用于高通量分析的微机械流动池(总尺寸为25x25x1 mm^3)。流动池由硅底芯片(0.5 mm厚)、两个穿孔(直径1 mm)和玻璃顶板(0.5 mm厚)组成。该通道(长50 cm，宽150μm，深20μm，通道间距150μm)由回火加工而成。两个0孔作为流体入口(中心)和出口(侧面)。芯片被放在光电倍增管的一个窗口前。透明的熔融石英毛细管(50μm.d.，375μm.D.)采用包被特氟龙AF的方法进行快速分析。在单线化学计量流动注射系统中，采用芯片和毛细管作为流动池。过氧化物酶(EC 1.11.1.7)被共价固定在通道内表面。一次测定的进样量为0.2min，最大进样率为315μ/h，载液流速为10μL/m in。H_20_2在5 nM-5μM范围内线性关系良好。用μ-Flow-Thry检测器测定了环境样品中的H_2O_2和生化样品中的奎达酶底物。

A MICROMACHINED FLOW CELL (OVERALL SIZE; 25x25x1 mm^3) WAS DESIGNED FOR HIGH THROUGHPUT ANALYSIS. THE FLOW CELL CONSISTED OF A SILICON BOTTOM CHIP (0.5mm THICK) WHICH HAD A SPIRAL CHANNEL (20 TURNS), TWO PIERCED HOLES (1mm DIAMETER), AND A GLASS TOP PLATE (0.5mm THICK). THE CHANNEL (50cm LENGTH, 150μm WIDTH, 20μm DEPTH, 150μm SPACING BETWEEN CHANNELS) WAS PRODUCED BY ECHING. TW0 HOLES SERVE AS THE FLUID INLET (CENTER) AND OUTLET (SIDE). THE CHIP WAS PLACED IN FRONT OF A WINDOW OF A PHOTOMULTIPLIER TUBE. ALSO, TRANSPARENT FUSED SILICA CAPILLARY (50μm I. D., 375μm O. D.) WHICH IS COATED WITH TEFLON AF WAS USED FOR MORE RAPID ANALYSIS. THE CHIP AND CAPILLARY WERE USED AS FLOW CELLS IN A SINGLE-LINE CHEMILUMINOMETRIC FLOW INJECTION SYSTEM. PEROXIDASE (EC 1.11.1.7) WAS COVALENTLY IMMOBILIZED ON THE INNER SURFACE OF THE CHANNEL. THE SAMPLE VOLUME FOR ONE MEASUREMENT WAS 0.2μL. THE MAXIMUM SAMPLING RATE WAS 315/h AT A CARRIER SOLUTION FLOW RATE OF 10μl/min. A CALIBRATION GRAPH FOR H_20_2 WAS LINEAR FOR 5 nM -5μM. THE SYSTEM WITH μ-FLOW-THROUGH DETECTOR WAS APPLIED TO THE DETERMINATIONS OF H_20_2 IN ENVIROMENTAL SAMPLES AND OF QXIDASE SUBSTRATES IN BIOCHEMICAL SAMPLES.