An evaluation of anti-VacA antibody and its target molecules in the cerebrospinal fluid of patients with demyelinating form of Guillain-Barre syndrome.

免疫学研究目的检测格林-巴利综合征（GBS）患者脑脊液中幽门螺杆菌重组空泡细胞毒素（r-VacA）抗体。对13例GBS患者（电生理学分类为急性炎性脱髓鞘性多根神经病变（AIDP） 8例、急性运动轴索神经病变（AMAN） 4例、不可兴奋性1例）和8例疾病对照患者的脑脊液样本进行了研究。采用SDS-PAGE分离r-VacA蛋白，并进行Western blot分析。13例GBS患者中有6例对幽门螺杆菌VacA有特异性IgG抗体，经r-VacA吸收实验证实。脑脊液抗r- vaca抗体阳性患者均有AIDP。VacA与人类(Na^++K^+)- atp酶α亚基的序列同源性进行了概述。我们的研究结果提出了一种可能的假设，即抗vaca抗体与轴突雪旺细胞质膜中的离子通道有关，导致一些GBS患者出现脱髓鞘。组织化学评价为鉴定人腹侧神经根抗vaca抗体（A-VacA-Ab）的靶分子，进行免疫组织化学检查。用幽门螺杆菌培养基纯化的天然VacA免疫制备兔源性A-VacA-Ab。颈腹神经根是在死后两小时内从无已知神经疾病的患者处获得的。采用DAB和FITC程序。横切面可见弥散斑状染色。在纵向切片上，DAB和FITC程序也可以看到串状免疫染色。这些都局限于神经纤维。因此，A-VacA-Ab的靶表位被认为存在于神经根的髓磷脂成分中。这些结果表明，GBS患者脑脊液中A-VacA-Ab的靶分子可能与周围神经髓磷脂的某些成分有关。A-VacA-Ab可能在脱髓鞘型GBS患者的免疫应答中起重要作用。少

Immunological studyIn order to detect antibodies against recombinant vacuolating cytotoxin (r-VacA) of Helicobacter pylori (H.pylori) in the cerebrospinal fluid (CSF) from patients with Guillain-Barre syndrome (GBS). The CSF samples from 13 patients with GBS (electrophysiologically classified as 8 acute inflammatory demyelinating polyradiculoneuropathy (AIDP), 4 acute motor axonal neuropathy (AMAN), and 1 unexcitable) and 8 disease control patients were studied. The r-VacA protein was separated by SDS-PAGE and Western blot analysis was carried out. Six of the 13 GBS patients had a specific IgG antibody against VacA of H.pylori, which was confirmed by absorption experiments using r-VacA. Every patient with positive CSF anti-r-VacA antibody had AIDP. The sequence homology between VacA and human (Na^++K^+)-ATPase α subunit has been outlined. Our findings lead to the possible hypothesis that anti-VacA antibodies involve ion channels in abaxonal Schwann cell plasmalemma resulting in demyeli … More nation in some patients with GBS.Histochemical evaluationTo identify the target molecules of anti-VacA-antibody (A-VacA-Ab) in the human ventral nerve roots, the immunohistochemical examination was carried out. A-VacA-Ab obtained from rabbits was prepared by immunization of the native VacA purified by the culture media of H.pylori. Cervical ventral nerve roots were obtained from patients with no known neurologic disorders within two hours after death. Both DAB and FITC procedures were employed. Disseminated spotty stainings were observed in cross sections. In longitudinal sections, string-like immunostainings were also seen both by DAB and FITC procedures. These were localized within the nerve fibers. Consequently, the target epitopes of A-VacA-Ab were thought to be present in the myelin components of the nerve roots. These results indicate that the target molecules of A-VacA-Ab in the cerebrospinal fluid obtained from patients with GBS are probably associated with some components of the peripheral nerve myelin. A-VacA-Ab may play an important role in the immune responses of patients with the demyelinating form of GBS. Less