The analysis of turbulent flow shear stress-responsive genes in vascular endothelial cells

应用DNA微阵列技术分析了层流和湍流切应力作用下人脐静脉内皮细胞（HUVECs）和冠状动脉内皮细胞（HCAECs）的基因表达。HUVEC和HCAEC中约3%的5600个基因在动脉水平的层流剪切应力（15达因/cm（2），持续24小时）下表达增加2倍以上或减少至静态对照的一半以下。在静脉水平的层流剪切应力（1.5达因/cm（2））下，两种细胞系中剪切应力反应基因的比例下降到2%左右。1.5 dynes/cm（2）的湍流剪切应力改变了HCAEC中1.1%的所有基因的表达。层流剪切应力，但不是湍流剪切应力，减少了一些基因的表达，参与DNA合成和细胞周期在HUVEC和HCAEC。聚类分析显示，HUVECs暴露于15 dynes/cm（2）的层流剪切应力3，6，12，24和48小时的基因表达的各种时间概况。湍流切应力影响了许多在血管重塑中发挥作用的基因的表达，包括编码纤溶酶原激活物及其抑制剂、内皮素-1、转化生长因子-β、IV型胶原蛋白和肝配蛋白A1的基因。

DNA microarray gene expression analysis was conducted in human umbilical vein endothelial cells (HUVECs) and coronary artery endothelial cells (HCAECs) exposed to laminar or turbulent shear stress. Approximately 3% of the total 5600 gene in HUVECs and HCAECs increased their expression more than two-fold or decreased it to less than half the static control in response to an arterial level of laminar shear stress (15 dynes/cm(2) for 24 hours). The proportions of shear-stress-responsive genes decreased to around 2% under the venous level of laminar shear stress (1.5 dynes/cm(2)) in both cell lines. Turbulent shear stress of 1.5 dynes/cm(2) altered the expression of 1.1% of all genes in the HCAECs. Laminar shear stress, but not turbulent shear stress, decreased the expression of a number of genes involved in DNA synthesis and the cell cycle in both HUVECs and HCAECs. Clustering analysis showed a variety of temporal profiles of gene expression in HUVECs exposed to laminar shear stress of 15 dynes/cm(2) for 3, 6, 12, 24, and 48 hours. Turbulent shear stress affected expression of many genes that play a role in vascular remodeling, including genes encoding plasminogen activators and their inhibitor, endothelin-1, transforming growth factor-beta, collagen type IV, and ephrin A1.