Peroxidase production using waste, and its use for the removal of phenolic compounds by polymerization and precipitation method

为降低过氧化物酶催化反应处理废水的成本，研究了利用廉价原料生产过氧化物酶的可行性。首先，从豆果酱和大米蒸煮过程中的废水被用作培养基。这些都不适合生产过氧化物酶。接下来，检查中等成分对较便宜成分的替代。原始培养基含有18 g/L葡萄糖、5g/L多肽和3g/L酵母提取物。其中，葡萄糖的量最大，酵母提取物的价格最高。虽然原糖和可溶性淀粉作为葡萄糖的替代品进行了检测，但两者都显示出相当少的过氧化物酶产生。另一方面，通过酶法降解金枪鱼罐头加工中的玉米渣而产生的细菌-N-KS作为葡萄糖的替代物是有效的。当添加1g/L Bacteria-N-KS代替酵母膏时，过氧化物酶的产率与原培养基基本相当，生产成本低于原培养基的1/3。双酚A、壬基酚和辛基酚在反应60分钟内容易地除去。适宜的反应条件为0-60℃、中性pH范围。通过测定过氧化物酶处理溶液的急性毒性和雌激素活性，可以清楚地看出，过氧化物酶处理不会产生有害产物。

Peroxidase production by Coprinus cinereus from an inexpensive materials was examined in order to reduce the cost of wastewater treatment by peroxidase-catalyzed reaction. First, the wastewaters from bean jam and rice cooking processes were used as the culture medium. These were not suitable for peroxidase production. Next, the substitution of the medium ingredients for the cheaper one was examined. The original medium contained 18 g/L glucose, 5 g/L Polypepton and 3 g/L yeast extract. Of these, the amount of glucose is the largest and the price of yeast extract is the highest. Although raw sugar and soluble starch were examined as the substitute of glucose, both showed quite little peroxidase production. On the other hand, Bacteria-N-KS that is produced by enzymatic degradation of tune residue in tuna can processing, was effective as the substitute of glucose. When Bacteria-N-KS was added at 1 g/L instead of yeast extract, the peroxidase productivity was almost the same level with that of the original medium, and the production cost of peroxidase was below one third that of the original medium.The removal of phenolic endocrine disruptors was then examined using the peroxidase obtained with the improved medium. Bisphenol A, nonylphenol and octylphenol were easily removed within 60 min of reaction. The suitable reaction conditions were 0-60℃ and neutral pH range. By the measurement of acute toxicity and estrogenic activity of the solution treated with peroxidase, it became clear that no harmful product was produced by the peroxidase treatment.