Studies on structural characterization and biological activities of diverse phosphogtycolipids in lower animals.

含肌醇磷脂(植物糖脂和/或微糖脂)低聚糖探针的结构分析(1)从海洋环节动物P.ochelata中分离纯化了三个新的含肌醇磷脂系列化合物。其化学结构完全确定为Ins-P-Cer，Manα1-6Ins-P-Cer，Manα1-6(Manα1-4)Ins-P-Cer。例如，这些化合物被鉴定为具有共同核心结构的半乳糖和分枝岩藻糖残基的新延伸结构(Manα1-2Ins-P-Cer)。(3)从丝状真菌Acremonum sp.的菌丝体中分离纯化了5种新的两性离子鞘糖脂。制备了识别磷脂分子中寡糖和/或磷酸基团的探针。从海洋环节动物(成虫)、异种丝虫中分离出抗α1-2 Ins-P-Cer和Manα1-2(Fucα1-5)Ins-P-Cer的抗血清，并对其化学结构进行了鉴定。这些脂类的神经酰胺部分由棕榈酸、人造硬脂酸和硬脂酸及其2-羟基酸为脂肪酸，鞘氨醇、Δ^&lt；4，8&gt；-鞘氨醇和9-甲基-Δ^&lt；4，8，10；-鞘氨醇为狮身人面像。

◎Structural analysis of oligosaccharide probes in glycosphingolipids containing inositolphosphate (phytoglycolipids and/or micoglycolipids)(1)Three novel series of glycosphingolipids containing inositolphosphate were isolated and purified from the marine annelid, P.ochelata. Their chemical structures completely characterized as Ins-P-Cer, Manα1-6Ins-P-Cer,Manα1-6(Manα1-4)Ins-P-Cer.(2)Mycoglycplipids were isolated from the seven edible fungi. For instance, these from Bunashimeji mushroom have been characterized as new extension structures of galactose and branched fucose residues with a common core structure (Manα1-2Ins-P-Cer).(3)Five novel zwitterionic glycosphingolipids have been isolated and purified from the mycelia of filamentous fungi, Acremonium sp. And also, a novel family of neutral glycosphingolipids was found in aureobasidin A-resistant Zygomycetes.◎Preparation of probes recognizing oligosaccharide and/or phosphate groups in phosphoglycolipid moleculesAntisera against Manα1-2Ins-P-Cer and Manα1-2(Fucα1-5)Ins-P-Cer isolated from the marine annelida (lugworm), T.heterochetus have been elicited in rabbits by repeated injection of a mixture of hapten-methylated bovine seraum albumin with Freund's adjuvant.◎Structural elucidation of sphingolipids from pearl oyster, P.martensiiSphingolipids, ceramide aminoethylphosphonate, sphingomyelin and cerebrosides were isolated and their chemical structures were characterized. The ceramide moieties of these lipids consisted of palmitic, margaric and stearic acids and their 2-hydroxy acids as fatty acids, and sphingosine, Δ^<4,8>-sphingadienine and 9-methyl-Δ^<4,8,10>-sphingatrienine as sphingoids.