Establishment of high-quality silage preparation technique corresponding to variation in sugar content of grass

1. 北海道基础草的水溶性碳水化合物（WSC）含量和糖组成随地区、生长期、收获时间和品种的不同而有显著差异。傍晚采收的禾草WSC含量高于早晨采收的禾草WSC含量，夜间枯草WSC含量降幅约为白天采收禾草WSC含量降幅的9倍。2 .添加乳酸菌接种剂的青贮饲料夜间萎蔫，在牧草含糖量低的地区品质较差。3 .采用CII组散射的mPLS方法制备的校正曲线，通过红外分析可以较准确地估计草中WSC的含量。低洼地和洼地禾草的水分含量与磷含量呈显著负相关，火山土禾草的水分含量与钾含量呈显著负相关。4 .禾草WSC含量与土壤中磷酸有效性、土壤速效钾之间无显著相关系数。干物质产量随施氮量的增加而增加，但WSC、果糖和蔗糖含量下降。增加施钾量对干物质产量和WSC含量没有影响。WSC含量晚草高于早草，夜枯草低于日枯草。晚草和夜枯草青贮发酵品质显著较高。收获期和萎蔫期的TDN和DE含量、DM和TDN摄入量均无显著差异。

1. The water-soluble carbohydrate (WSC) content and sugar composition of the basic grass in Hokkaido, timothy, markedly varied depending on the area, growth stage, harvest time, and breed.2. The WSC content of grass harvested in the evening was higher than that harvested in the morning, and the reduction of the WSC content of grass wilted at night was about 9 times greater than that of grass wilted in the daytime. Lactic acid bacterial inoculant-added silage wilted at night showed a poor quality in regions in which the sugar content of grass was low.3. The WSC content of grass could be estimated with practical accuracy by infrared analysis employing a calibration curve prepared by the mPLS method with scatter using the CII group.4. A significant negative correlation was noted between the WSC and P contents of grass grown in low and table land soils, and between the WSC and K contents of grass grown in volcanic soil. No significant correlation coefficient was obtained between the WSC content of grass and the availability of phosphoric acid in soil or between the WSC content and available soil potassium, regardless of the soil type.5. The dry matter yield increased to the standard application rate as the nitrogen application rate increased, but the WSC, fructose, and sucrose contents decreased. The dry matter yield or WSC content could not be changed by increasing the potassium application rate.6. The WSC content was higher in the evening grass than in the morning grass, and lower in the night-wilted than in daytime-wilted grass. The fermentation quality of silage was significantly higher in the evening grass and night-wilted grass. There were no significant differences in the TDN or DE content or in the DM or TDN intake between the harvest or wilting times.