ANALYSIS OF ADHESIVE AND INVASION MECHANISM OF PIROPLASMA IN VECTOR TICK

1.研究了针注法作为将马巴氏巴贝虫和马巴氏巴贝虫感染长角血蜱的实验方法的有效性。此外，我们还确定调查这些寄生虫在这种蜱的跨卵和跨stadial传播。通过皮下注射针将B.caballi或B. equi感染的红细胞从体壁注射到充血的成虫或若虫蜱的中肠中。采用巢式PCR检测蜱体内马巴贝虫DNA。对于跨卵传播，分别在成蜱、产卵和幼虫中检测到B.caballi和B.equi DNA。对于经尿道传播，B。在注射若虫蜕皮后的成蜱中以及注射若虫蜕皮后的成蜱唾液腺和尸体组织中检测到马DNA。这些结果支持长角血蜱在马巴贝西亚传播中的潜在作用。2.建立了针对马巴贝西亚表面蛋白和裂殖子的单克隆抗体，并利用其筛选从巴氏裂殖子mRNA制备的cDNA表达文库中的高表达蛋白。结果表明，PCR方法在实验感染中具有良好的重复性和准确性，且灵敏度远高于传统的显微镜检查。4.Apo-LF对B.caballi的抑制作用最强，这可能是由于灭活或抑制了培养液中的某种生长因子所致。

1.We investigated the usefulness of the needle injection method as an experimental means of transferring infection of equine Babesia parasites, namely Babesia caballi and Babesia equi, to the tick Haemapysalis longicornis. Further, we also determine investigated the transovarial and transstadial transmission of these parasites in this tick. Engorged adult or nymph ticks were injected with B.caballi- or B.equi-infected erythrocytes into the midgut from the integument by hypodermic needle. Detection of equine Babesia in ticks was by nested PCR for parasite DNA. For transovarial transmission, B.caballi and B.equi DNA were detected in adult ticks, laid eggs and larvae, respectively. For transstadial transmission, B. equi DNA was detected in adult ticks after molt of the injected nymph, and in salivary glands and carcass tissues of adult ticks after molt of the injected nymph. These findings support the potential role of H.longicornis in the transmission of equine Babesia.2.We established mAbs against the Babesia surface protein and extracellular merozoite termed mAb, and used it to screen cDNA expression library prepared from Babesia merozoite mRNA for highly expressed proteins.3.Real time-PCR was found to be reproducible and accurate for the quantification of parasite DNA in experimentally infection and far more sensitive than traditional microscopic examination. LAMP method provides a useful tool for the detecton of B.gibsoni infection in dog.4.Apo-LF had the strongest inhibitory effect on B.caballi ; this may have been caused by inactivation or inhibition of a growth factor in the culture medium.