On-site Analysis of the Endocrine-disrupting Alkylphenolic Compounds Based upon the Redox Recycling at the Applied Potential of 0 V

提出了环境水体中干扰内分泌的烷基酚类化合物的现场分析系统。该系统由烷基链包覆电极上氧化还原循环对二茂铁衍生物的安培检测、固相衍生化结合固相萃取环境水和微尺度分离组成。基于氧化还原循环的二茂铁衍生物的电流检测提出了一种基于二茂铁酚的还原态和氧化态与亚铁氰化物和硝酸铁反应连续再生的灵敏检测方法，分别用于内分泌干扰型烷基酚类化合物的HPLC分析。在氧化电流放大的情况下，检测限为10^<-12> mol/L水平。然而，在施加电位为0 V时，减小电流的放大比约为10.2。烷基酚类化合物的固相衍生化研究了干扰内分泌的烷基酚类化合物在C_<18>改性硅胶（ODS）上取代有机溶剂的甲酰化和丹酰化反应，目的是建立一种环保高效的衍生化分析方法。固相丹基化系统已被纳入通过从自来水中固相萃取进行的预浓缩步骤。即使在浓度小于10^<-11> mol/L的情况下，富集后的酚类也能有效地丹化，并得到清晰的色谱图。固相衍生化适用于涉及转化为由磺酰基连接的可检测产物的其他体系。干扰内分泌的烷基酚类化合物现场分析系统的提出我提出了一种由烷基链包被电极氧化还原回收的二茂铁基衍生物的安培检测、固相衍生化结合环境水固相萃取和微尺度分离组成的现场分析系统。少

The on-site analytical system for the endocrine-disrupting alkylphenolic compounds in environmental water has been proposed. The system consists of amperometric detection of the ferrocenoyl derivatives using the redox recycling on an alkyl chain-coated electrode, the solid-phase derivatization combined with the solid-phase extraction of environmental water, and the micro-scale separation.1. Amperometric Detection of the Ferrocenoyl Derivatives Based on the Redox RecyclingA sensitive detection method based upon continuous regeneration of the reduced and oxidized forms of the ferrocenoyl phenols by reaction with ferrocyanide and ferric nitrate is proposed for HPLC analyses of endocrine disruptive alkylphenolic compounds, respectively. In the case of the amplification of the oxidation current the detection limit is the 10^<-12> mol/L level. However, the amplification ratio for the reduction current at the applied potential of 0 V is about 10.2. Solid-phase Derivatization of the Alkylpheno … More lic CompoundsThe tosylation and dansylation reactions of the endocrine-disruptive alkylphenolic compounds on a C_<18>-modified silica gel (ODS) instead of organic solvents were investigated with the aim of developing an enviromnent-friendly and efficient derivatization method for the HPLC analyses. The solid-phase aqueous dansylation system has been incorporated into a preconcentration step performed by solid-phase extraction from tap water. Even the phenols at concentrations less than 10^<-11> mol/L can be efficiently dansylated after enrichment and give a clear chromatogram. The solid-phase derivatization is applicable to the other systems involving conversion into the detectable products inked by the sulfonyl group.3. Proposal of the On-site Analytical System for the Endocrine-Disrupting Alkylphenolic CompoundsI have proposed the on-site analytical system consisting of amperometric detection of the ferrocenoyl derivatives using the redox recycling on an alkyl chain-coated electrode, the solid-phase derivatization combined with the solid-phase extraction of environmental water, and the micro-scale separation. Less