Study of antiviral and anticancer effects of polyphenols

1)抗病毒研究(1)抗HTV检测：用HIV-I感染MT-4细胞，并与不同浓度的受试化合物孵育。孵育5d后，用四甲基偶氮唑盐比色法测定细胞活力，以评价受试化合物对HIV诱导的CPE的抑制活性。(2)抗HSV实验：将HSV-1感染的Vero细胞与受试化合物共同孵育，以抑制病毒诱导的CPE活性为指标。2)细胞毒活性研究(1)细胞毒活性测定：3种口腔肿瘤细胞系(HSG、HSC-2、HSC-3)和3种正常细胞(HGF、HPC、HPLF)与受试化合物共同孵育。培养24小时后，用mtt法测定相对活细胞数。(2)dna片段化检测：用受试化合物…处理培养细胞用含逆转录病毒的mdr1/A基因转染小鼠T细胞淋巴瘤细胞株L5179，筛选出表达mdr1基因的细胞。通过对B16-BL6黑色素瘤细胞的实验性肺转移实验，研究了化合物的逆转活性。(4)用ESR波谱分析了Caspase的活性和抗自由基活性。(5)通过对B16-BL6黑色素瘤细胞的实验性肺转移实验，研究了几种植物的提取物对黑色素瘤的抑制作用。托波龙衍生物由于自由基介导的氧化还原反应而具有抗癌活性。4F-苯甲酰基-TE14011是合成肽T22和CXCR4拮抗剂的类似物，被一种可生物降解的聚合物聚D，L-乳酸包裹。皮下单次注射4F-苯甲酰基-TE14011-聚乳酸可显著减少B16-BL6黑色素瘤细胞肺转移形成的集落数量。较少

1)Antiviral study(1)Anti-HTV assay : MT-4 cells were infected with HIV-I and incubated in the presence of various concentrations of test compounds. After incubation for 5 days, cell viability was quantified by MTT assay to estimate a inhibition activity of test compounds against HIV induced CPE. Test compounds were alkyl-ologosaccharides, synthetic peptides, and an extract from, an Indian medical plant, Indigofera cordifora.(2)Anti-HSV assay : HSV-1 infected Vero cells were incubated in the presence of test compounds and antiviral activity was evaluated as the inhibition activity against virus induced CPE.2)Study for anticancer activities(1)Assay for cytotoxic activity : Three human oral tumor cell lines, (HSG, HSC-2, HSC-3), and 3 human normal cells, (HGF, HPC, HPLF) were incubated in the presence of test compounds. After 24 h incubation, the relative viable cell number was detenraned by MTT assay.(2)Assay for DNA fragmentation : DNA fragmentation from cultured cells with test compoun … More ds treatment was determined to estimate the inhibitory activity against apoptosis.(3)L5179 mouse T cell lymphoma cell line was transfected with MDR1/A containing retrovirus and MDR1 expressing cells were selected. The reversed activity of test compounds were studied.(4)Caspase activation and anti-radical activity using by ESR spectroscopy were also carried out.(5)Anti-melanoma metastatic activity was assessed using an experimental pulmonary metastasis assay of B16-BL6 melanoma cells.According to the results of these experiments, extracts from several plants, such as Anastasia Red, demonstrate anticancer activity and inhibition of MDR expression. Tropolone derivatives have anticancer activity due to radical mediated redox reaction. 4F-benzoyl-TE14011,an analog of synthetic peptide T22 and CXCR4 antagonist, was encapsulating with a biodegradable polymer, poly D, L-lactic acid (PLA). Subcutaneous single administration of 4F-benzoyl-TE14011-PLA significantly reduced the number of colonies formed by pulmonary metastasis of B16-BL6 melanoma cells. Less