BIOLOGICAL CHARACTERISTICS OF PRIMARY MAMMARY MALIGNANT LYMPHOMA

我们分析了原发性乳腺弥漫性大B细胞淋巴瘤（DLBCL），I期和II期，以揭示为什么原发性乳腺DLBCL表现出较差的扩散。石蜡包埋切片免疫组化：淋巴瘤细胞免疫表型如下：CD 3;0/15（阳性例/检查例），CD 5; 2/15，CD 10; 0/15，CD 20; 15/15，Bcl-2; 10/15，Bcl-6; 5/15和IRF 4/MUM 1; 15/15。MIB-1增殖指数为59.5%~ 95.2%，平均为79.2%。免疫球蛋白重链可变区（IgH-V）突变分析：7例受检者IgH-V突变频率分布在1.43%~ 10.29%之间。结果表明，乳腺DLBCL的淋巴瘤细胞属于CD 10 ^- Bcl-6^<+/-> IRF 4/MUM 1 ^+非生殖中心B细胞DLBCL（活化B细胞DLBCL）亚群，并具有较高比例的MIB-1增殖指数。乳腺DLBCL患者预后较差可能与淋巴瘤细胞MIB指数高有关.为了找到乳腺DLBCL的淋巴瘤细胞具有高MIB-1增殖指数的原因，使用人10 K寡核苷酸对4例乳腺DLBCL和4例结DLBCL进行了cDNA微阵列分析。8例患者均为30岁以上女性。所有病例淋巴瘤组织学均为中心母细胞型DLBCL，免疫组化均为非生殖中心B细胞型DLBCL。发现了一些基因在两组间有不同的表达模式，我们发现了原发性乳腺DLBCL的生物学特征，并对这些基因进行了详细的分析。有或无Bcl-6表达的病例可能是重要的。

We analyzed primary mammary diffuse large B-cell lymphoma (DLBCL), with stage I and II, in order to disclose why primary mammary DLBCL showed a poorer prognosis.1. Immunohistochemistry of the paraffin-embedded section : Immunophenotype of the lymphoma cells is as follows: CD3;0/15 (positive cases/examined cases), CD5; 2/15, CD10; 0/15, CD20; 15/15, Bcl-2; 10/15, Bcl-6; 5/15 and IRF4/MUM1; 15/15. A MIB-1 proliferation index was from 59.5% to 95.2% with an average of 79.2%.2. Mutation analysis of the immunoglobulin heavy chain variable region (IgH-V): Mutation frequency of examined 7 cases distributed from 1.43 to 10.29%. No ongoing mutation was found in examined 5 cases.These results, therefore, indicated that the lymphoma cells of mammary DLBCL belonged to a subgroup of CD10^- Bcl-6^<+/-> IRF4/MUM1^+ non-germinal center B cell DLBCL (activated B-cell DLBCL) and exhibited a high proportion of MIB-1 proliferation index. A poorer outcome of patients with mammary DLBCL may be related to high MIB index of the lymphoma cells.3. cDNA microarray using a human 10K oligotip was analyzed between 4 cases of mammary DLBCL and 4 cases of nodal DLBCL in order to find the reason why the lymphoma cells of mammary DLBCL have a high MIB-1 proliferation index. The 8 patients were all female and more than 30 years old. The lymphoma of all cases showed a centroblastic variant of DLBCL in histology and non-germinal center B cell DLBCL by immunohistochemistry. Some genes having a different expression pattern between two groups were found.We found biological characteristics of primary mammary DLBCL and continue to analyze details of the genes. Cases with or without Bcl-6 expression may be important.