Effect of thyroid hormone on the production of cytokines from lymphocytes

Graves病患者甲状腺功能恢复正常后，TSH受体抗体值下降。破坏性甲状腺毒症的破坏持续时间少于三个月。为了解释这些现象，检验了“甲状腺激素抑制Th 1并刺激Th 2”的假设。结果：（1）全血与甲状腺激素（T3）一起培养，用侵入RNA测定法测定IFN-γ和IL-4的含量。T3轻微增加IFN-γ和轻度增加IL-4。(2)采用TaqMan Cytokine Gene Expression Plate I检测4例受试者外周血单个核细胞中几种细胞因子的表达。不同标本的结果不同。(3)用PHA和T3培养全血，通过ELISA测定上清液中IL-4的量。T3对IL-4无明显影响。(4)用PHA和T3培养外周血单个核细胞，用ELISPOT检测IFN-γ和IL-4产生细胞。T3轻微增加IFN-γ产生细胞，轻微减少IL-4产生细胞。结论：甲状腺激素在淋巴细胞产生细胞因子方面没有表现出恒定的变化。

The value of TSH receptor antibody will fall if thyroid function is normalized in Graves' disease. The duration of destruction is less than three months in destructive thyrotoxicosis. In order to explain these phenomenon, the hypothesis "thyroid hormone suppresses Th1 and stimulates Th2" was examined. Results ; (1) Whole blood was cultured with thyroid hormone (T3), and the amounts of IFN-gamma and IL-4 were measured with Invader RNA Assay. T3 slightly increased IFN-gamma and mildly increased IL-4. (2) Peripheral blood mononuclear cells from four people were cultured with T3, and the amounts of several cytokines were measured with TaqMan Cytokine Gene Expression Plate I. The results were different in every specimen. (3) Whole blood was cultured with PHA and T3, and the amount of IL-4 in a supernatant was measured by ELISA. IL-4 was not changed significantly by T3. (4) Peripheral blood mononuclear cells were cultured with PHA and T3, and the IFN-gamma or IL-4 producing cells were measured by ELISPOT. T3 slightly increased IFN-gamma producing cells, and slightly decreased IL-4 producing cells. Conclusions ; Thyroid hormone did not show a constant change in the Cytokine production from lymphocytes.