In vivo differentiation and proliferation of mesenchymal stem cells derived from cord blood stem cell transplantation donor.

研究背景：骨髓间充质干细胞在体外培养体系中可分化为骨和软骨细胞。与成人骨髓相比，人脐血中含有丰富的间充质干细胞。分析脐血干细胞移植供体来源的骨髓间充质干细胞(MSC)在体内分化和增殖的可能性。1)供体细胞来源的骨和软骨细胞分析一例家族性噬血细胞淋巴组织细胞增多症(FHL)婴儿接受了脐血干细胞移植。在供体细胞植入后，发现他的头骨有骨溶解，并进行了骨活检。以20号染色体G08062为靶点，用聚合酶链式反应方法分析标本中是否含有供体(脐血干细胞)来源的MSC。结果显示，供体来源的细胞中有70%为混合嵌合体。2)供体细胞(脐血干细胞)来源的骨髓细胞分析1例6岁难治性Burkitt淋巴瘤患儿接受了人类白细胞抗原2(HLA2)不相合供者脐血干细胞移植。以18、20、22号染色体上的微卫星标记和人类白细胞抗原差异为靶点，采用聚合酶链式反应方法分析移植后不同细胞系骨髓嵌合体的变化。不幸的是，移植后不久就发现了骨髓移植，所以结果显示了100%的受者来源细胞。

Research Background : Mesenchymal stem cells in bone marrow have been known to differentiate into bone and cartilage cells in vitro culture system. Human cord blood is abundant with the mesenchymal stem cells in comparison with adult bone marrow. We analyzed the possibility of differentiation and proliferation of mesenchymal stem cell(MSC) in vivo, which derived from cord blood stem cell transplantation donor.1)Analysis of bone and cartilage cells derived from donor cells (cord blood stem cell)An infant with familial hemophagocytic lymphohistiocytosis(FHL) received a cord blood stem cell transplantation. After the engraftment of the donor cells occurred, osteolysis was found in his skull and bone biopsy was done. We analyzed whether the specimen include the donor (cord blood stem cell) derived MSC or not, using the PCR method targeting the G08062 on the chromosome 20. The results revealed the mixed chimerism with 70% of donor derived cells. Then we prepared the single cell PCR method to analyze the origin of single cell extracted by the microdissection method, but it was difficult to get the enough DNA from the bone tissue for the analyses.2)Analysis of bone marrow cells derived from donor cells (cord blood stem cell)A 6-year-old boy with refractory Burkitt lymphoma received a cord blood stem cell transplantation from HLA 2 loci mismatched donor. We analyzed how the chimerism varied in the different cell lineage of bone marrow after the transplantation, using the PCR method targeting the microsatellite markers on the chromosome 18,20,22 and HLA disparity. Unfortunately, the bone marrow relaplse was found soon after the transplantation, so the results revealed the 100% of recipient derived cells.