Association of allergic disorders and FOXP3, the responsible gene for X-linked autoimmunity-allergic disregulation syndrome (XLAAD).

1.采用羧荧光素琥珀酰亚胺酯（CFSE）稀释法，对牛奶过敏（CMA）患儿的牛奶蛋白（CMP）特异性t细胞增殖和细胞因子产生进行了分析。观察CMA患儿与非过敏患儿cmp特异性CD4+细胞增殖及TH1/TH2细胞因子产生的差异。虽然最近摆脱CMA的儿童的增殖反应与患者没有显着差异，但细胞因子产生的模式与非过敏儿童相似。CMA患儿CD4+IL-10+调节性T细胞增殖与非过敏患儿无显著差异。FOXP3基因与过敏性疾病的相关性研究。采用ABI PRISM 3100基因分析仪分析FOXP3基因零内含子上的微卫星多态性（GT）n，并对FOXP3基因与食物过敏的关系进行研究。（GT）_<16>等位基因在食物过敏患者中的出现频率明显低于对照组，提示FOXP3基因可能影响食物过敏的严重程度。

1.Allergen-specific helper T-cell response in patients with cow's milk allergyBy using carboxyfluorescein succinimidyl ester (CFSE) dilution assay, both cow's milk protein (CMP)-specific T-cell proliferation and cytokine production were analyzed simultaneously in children with cow's milk allergy (CMA). Significant differences of both CMP-specific CD4+ cell proliferation and TH1/TH2 cytokine production between CMA and non-allergic children were observed. While the proliferative responses of children who recently outgrew CMA were not significantly different from those of patients, the patterns of cytokine production were similar to those of non-allergic children. Proliferation of CD4+IL-10+ regulatory T cell was not different between CMA and non-allergic children.2.An association study between the FOXP3 gene and allergic disorders.A microsatellite polymorphisms, (GT)n, located on intron zero of FOXP3 gene was analysed by ABI PRISM 3100 Genetic Analyzer and an association study between the FOXP3 gene and food allergy were performed. The (GT)_<16> allele showed a significantly lower frequency in patients with food anaphylasxis than in controls, suggesting the possibility that FOXP3 gene may affect severity of food allergy.