Interaction between Iopamidol and Gadopentetate Dimeglumine : An in-vitro experimental study of direct mixing

目的：采用直接混合法测定碘帕醇与钆喷酸葡胺在试管中是否存在相互作用材料和方法：将碘帕醇（Iopamiron 300）和钆喷酸葡胺（Magnevist）以1：1的比例混合制备供试品溶液。在混合后立即和1、3、6和24小时评估混合溶液的颜色、粘度和pH。测定混合溶液中芳香族伯胺的浓度、碘帕醇的含量、游离碘离子的浓度和Gd-DTPA的含量，并使用薄层色谱法测定是否存在除碘帕醇和Gd-DTPA产生的斑点以外的斑点。这些测试在混合后立即和24小时进行。结果：直接混合后即刻、1.3、6、24 h，碘帕醇与Gd-DTPA混合液的外观及pH值均无明显变化。结论：碘帕醇与钆喷酸葡胺在试管中直接混合后无明显的相互作用。

Objective : To determine whether or not there is any interaction between iopamidol and Gd-DTPA in test tubes using the direct mixing manner.Materials and Methods : The test solution was prepared by mixing iopamidol (Iopamiron 300) and Gd-DTPA (Magnevist) at a ratio of 1:1. The color, viscosity, and pH of the mixed solutions were assessed immediately and 1,3,6,and 24 hours after mixing. The concentration of aromatic primary amines, content of iopamidol, concentration of free iodine ion, and content of Gd-DTPA in the mixed solution were determined, and presence or absence of spots, other than those resulting from iopamidol and Gd-DTPA, was determined using thin layer chromatography. These tests were carried out immediately and 24 hours after mixing. Using iopamidol alone and Gd-DTPA alone as controls, the same items were examined.Results : There was no significant change in the appearance or pH of the mixed iopamidol and Gd-DTPA solution immediately and 1.3,6,and 24 hours after direct mixing. The study of iopamidol alone and Gd-DTPA alone showed no apparently abnormal values or findings.Conclusion : The direct mixing of iopamidol and Gd-DTPA in a test tube results in no significant interaction.