Characterization of clinically useful human monoclonal antibodies against cancers of digestive organ origin.

目的：SKI是一种人IgM单克隆抗体，可识别被称为AgSK1的抗原，该抗原被证明在人腺癌，特别是人类胃肠道恶性肿瘤中优先表达。本研究的目的是阐明AgSK1 mRNA表达在人类结直肠癌中的临床病理意义。方法：采用定量RT-PCR技术，研究40例结直肠癌患者切除标本中AgSK1 mRNA的表达水平。我们估计肿瘤组织值（T值）、非肿瘤组织值（N值）和T值与N值之比（T/N ratio）。为了进一步分析，我们将这些数据与临床病理特征进行了比较。结果：在淋巴结转移状态下，pN阳性状态的AgSK1 mRNA的t值水平往往高于pN阴性状态（P=0.076）。根据平均T值（8.032）或平均T/N比（1.549）将患者分为低表达组和高表达组。高表达组淋巴结转移阳性发生率显著高于对照组（T值P=0.021，T/N比P=0.024）。结论：肿瘤组织中AgSK1 mRNA的表达可能成为结直肠癌淋巴结转移的有用标志物和恶性潜在标志物。

PURPOSE : SKI, a human IgM monoclonal antibody recognizes the antigen, termed AgSK1 which was shown to be preferentially expressed by human adenocarcinomas, particularly human gastrointestinal malignancies. The aim of this study was to clarify the clinicopathological significance of AgSK1 mRNA expression in human colorectal cancer. METHODS : Using a quantitative RT-PCR, we studied the mRNA expression level of AgSK1 in the resected specimen of 40 patients with colorectal cancer. We estimated the tumor tissue value (T-value), nontumorous tissue value (N-value), and the ratio of T-value to N-value (T/N Ratio). For further analysis, we compared these data with the clinicopathological features. RESULTS : In the status of lymph node metastases, pN positive status tended to have a higher T-value level of AgSK1 mRNA than pN negative status (P=0.076). According to a mean T-value (8.032) or a mean T/N Ratio (1.549), we divided these patients into two groups, low expression group and high expression group. A high expression group showed a significantly higher frequency of positive lymph node metastases (T-value ; P=0.021,T/N Ratio ; P=0.024). CONCLUSION : AgSK1 mRNA expression in tumor tissue may become a useful marker for lymph node metastases and a malignant potential marker of colorectal cancer.