Targeted radio-immuno-chemotherapy against liver metastases of gastric cancer using radio-labeled chimeric monoclonal antibody and chimeric antibody-drug conjugates.

1)放射性标记嵌合单克隆抗体的制备嵌合单克隆抗体chA7用131- 1标记并纯化。2) 131-I标记chA7的体外抗肿瘤作用研究131-I标记chA7对人胃癌细胞株的体外抗肿瘤作用。其作用大于单独使用chA7。3)人胃癌细胞系制备小鼠肝转移瘤模型采用人胃癌细胞系MKN1、MKN45、KATOIII注入脾浆膜下制备小鼠胃癌肝转移瘤模型。没有成功地生产出合适的型号。4) 131-I标记chA7对小鼠的急性毒性检查131-I标记chA7对小鼠的急性毒性。在55.5MBq/a小鼠浓度下，131-I chA7无严重毒性。7.5)131-I chA7在胃癌肝转移小鼠体内的生物分布由于无法建立小鼠肝转移模型，故未对131-I chA7在胃癌肝转移小鼠体内的生物分布进行研究。131- icha7在小鼠胃癌皮下肿瘤中的生物分布。131- icha7在注射后2小时积聚最多。6) 131-I chA7对胃癌的体内杀瘤作用。肿瘤接种2周后，131-I chA7分别给予7.4MBq/只小鼠和14.8MBq/只小鼠。两组均无明显疗效。

1)Preparation of radio-labeled chimeric monoclonal antibodyChimeric monoclonal antibody chA7 was labeled with 131-I, and purified.2)In vitro antitumor effects of 131-I labeled chA7In vitro antitumor effects of 131-I labeled chA7 against human gastric cancer cell lines were investigated. The effects showed greater than that of chA7 alone.3)Production of murine liver metastases model using human gastric cancer cell linesA murine liver metastases model of gastric cancer was tried to produce using human gastric cancer cell lines, such as MKN1, MKN45 and KATOIII, injected into subserosa of spleen. A suitable model was not succeeded to produce.4)Acute toxicity of 131-I labeled chA7 for miceAcute toxicity of 131-I labeled chA7 for mice was checked. No severe toxicity was noted under 55.5MBq/a mouse of 131-I chA7.5)Biodistribution of 131-I chA7 in mice bearing liver metastases of gastric cancerBiodistribution of 131-I chA7 in mice bearing liver metastases of gastric cancer was not investigated because murine liver metastases model could not be made. So biodistribution of 131-I chA7 in mice bearing subcutaneous gastric cancer tumor. 131-I chA7 accumulated most in the tumor 2 hour after injection.6)In vivo tumoricidal effects of 131-I chA7 against gastric cancerNude mice bearing human gastric cancer tumor were received 7.4MBq/ a mouse or 14.8MBq/ a mouse of 131-I chA7 2 weeks after tumor inoculation. No significnant effect was noted in both group.