Establishment of the rat glioma model at the midbrain and screenig of the tumor suppressor function

由于治疗人类脑干胶质瘤仍然具有挑战性，需要实验胶质瘤模型。我们开发了一种大鼠内源性脑干胶质瘤模型，该模型使用立体定向框架来固定头部，以通过永久植入的套管将C6胶质瘤细胞递送到靶位点。我们将5 x 10^4个细胞悬浮在1 μl培养基中，接种大鼠中脑、脑桥或大脑皮层2 min。植入后3天，在中脑和脑桥被盖的导水管周围灰质中可见肿瘤形成。在第10<th>天，肿瘤直径超过2 mm ;长度，没有肿瘤细胞种植到脑脊液空间中。肿瘤表现出典型的胶质母细胞瘤的组织学特征; Ki-67标记指数为32%。因为在我们的模型中，套管是永久植入的，所以可以输送额外的接种物。最后，我们建立了立体定向准确的脑干（包括中脑）生殖性胶质瘤模型。

As the treatment of human intrinsic brainstem gliomas remains challenging, experimental glioma models are needed. We developed a rat model of intrinsic brain stem glioma that uses a stereotactic frame to fix the head for the delivery of C6 glioma cells to target sites via a permanently implanted cannula. We inoculated the rat midbrain, pons, or cerebral cortex with 5 x 10^4 cells suspended in 1 μl of culture medium over the course of 2 min. Three days post-implantation, tumor formation was visible in the periaqueductal gray matter in the midbrain and in the tegmentum of the pons. On the 10^<th> day, the tumor diameter exceeded over 2 mm ; length, there was no tumor-cell seeding into the cerebrospinal fluid space. The tumor manifested the histological features typical of glioblastoma ; Ki-67 labeling index was 32%. Because in our model the cannula is permanently implanted, additional inocula can be delivered. Finally, we establish the reproductive glioma model at the brain stem including midbrain with stereotactically accuracy.