The approach for bone regeneration using PRP (Platelet-Rich Plasma). Morphological observation of microcirculation after alveolar bone adaptation.

前言骨再生治疗已通过多种技术手段进行了研究。目前认为PRP可促进血管生成和骨再生。本研究在扫描电子显微镜(SEM)下观察了PRP人工骨移植后的微血管变化和骨形成。微血管树脂铸型是一种观察血管系统的方法。通过向毛细血管完全注入合成树脂，可以观察到清晰的三维图像。材料和方法6只比格犬用于实验动物。拔除双侧4颗前磨牙的下颌骨。将拔除的牙槽窝分成4组。一侧牙槽窝应用PRP和人工合成植骨材料(磷酸三钙)。结果与讨论拔牙组应用PRP于牙槽窝可促进血管再生。在这些血管之间形成了一块新的骨。然而，如果没有支架，PRP无法恢复骨高度。应用PRP的TCP移植组，TCP腺周围可见丰富的血管再生。明显促进了磷酸三钙的吸收。复制骨小梁。由于磷酸三钙起到了支架的作用，骨高度得以恢复。结论PRP的应用极大地促进了骨再生。据了解，PRP促进血管和骨骼的再生。

IntroductionBone regeneration therapy has been examined by various techniques. It is considered that PRP promotes angiogenesis and bone regeneration. In this study, microvascular changes and bone formation after synthetic graft using PRP was examined under a scanning electronic microscope (SEM). Microvascular resin cast is a method to observe the vasculature. A clear, three-dimensional image can be observed through the complete infusion of a injected synthetic resin up to the capillaries.Materials and MethodsSix beagle dogs were used to experimental animals. The mandiblal bone of premolars of 4 teeth on both sides were extracted. These extracted sockets were divided into 4 groups. PRP and synthetic bone graft material (TCP ; Tricaluciumphosphate) was applied to the sockets of one side. One side is used to the control.Results and DiscussionIn the tooth extraction group, vascular regeneration is promoted by applying PRP to tooth socket. A newly formed bone had occurred between these blood vessels. However, without scaffold, PRP doesn't recover the bone height. In the TCP graft group of with the application of PRP, regeneration of rich blood vessel was seen around the TCP glanules. The absorption of the TCP was advanced clearly. The trabeculae was reproducted. Because TCP worked for scaffold, bone height was recovered.ConclusionThese results suggest that application of PRP greatly advanced the bone regeneration. It is understood that PRP promotes the regeneration of the blood vessels and the bone.