The regeneration of tooth in combination with homologous transplantation of freezed tooth germ and non-freezed bone marrow tissue.

将硬组织形成初期的未成熟牙胚移植到牙槽内组织的研究很少，而将这些组织移植到肾囊或背部皮肤皮下组织的研究也有类似的研究。首先在光镜下观察冷冻牙胚的细胞形态和凝集素免疫组化，探讨冷冻保存牙胚对牙胚各细胞成分的影响。同时对复合多糖进行光镜染色，观察到这些多糖大量存在于细胞间组织中。将大鼠上第一磨牙胚于出生后5天冷冻保存，1周后解冻保存，并与未冷冻的对照牙胚在成釉细胞、中间层细胞、牙髓星状细胞、成牙髓细胞、牙髓细胞和血管内皮细胞上的组织学和复合多糖的表达进行比较。随后，复方多糖的组织学、凝集素表达和染色结果显示，实验组和对照组在大多数细胞和细胞间成分上没有明显差异，表明冷冻保存对牙胚组织没有明显影响。因此，我们从一半的兄弟姐妹中取出出生后5天的牙胚，冷冻保存，并在出生后13天移植到其余兄弟姐妹的颌骨中，并在术后1周和2周组织学上探讨移植的牙胚形成硬组织的能力。糖链和复合多糖的染色也与未冷冻对照组进行了比较。术后1周散在观察到新牙凹形成，术后2周牙本质变厚。糖链和复合多糖在牙髓细胞和成牙本质细胞的表达和定位上与对照组没有明显的差异。上述结果提示冷冻牙胚维持了牙本质的发生。冻僵的牙胚发生牙釉质吸收或骨性强直，这两种情况均可发生在牙胚外伤中。牙胚移植结合胫骨骨髓组织的植入，形成了稍厚的牙本质，虽然植入后残留的骨髓不能确定，但这种骨髓组织可能与改善的牙本质形成有关，如果有的话。少

Very few researches were available on the transplantation of immature tooth germs at the initial stage of hard tissue formation into intraalveolar tissue, although similar studies on the transplantaion of those tissues into the capsule of kidney or the subcutaneous tissues of the back skin. Initially, cell morphology and lectin immunohistochemistry were examined in freezed tooth germ with light microscopy, to explore whether preservation of tooth germ by freezing could make any influence on various cellular components of tooth germ. Simultaneously, light microscopic staining of compound polysaccharides were carried out to observe these polysaccharides which exists abundantly at the intercellular tissue.The rat tooth germ of upper first molar was preserved by freezing at postnatal 5 day, thawed after one week, and histology and the expression of compound polysaccharides were compared with non-freezed control tooth germ on ameloblasts, cells of the intermediate layer, stellate cells of e … More namel pulp, odontoblasts, pulp cells and vascular endothelial cells. Subsequently, histology, lectin expression and staining of compound polysaccharides showed no definite difference in most of cellular and intercellular components between the experimental group and the control, suggesting that preservation by freezing made no significant changes in tooth germ tissue.Consequently, tooth germs at postnatal 5 days were taken from the half number of siblings, and preserved by freezing, and transplanted into the jaw bone of the remaining siblings at postnatal 13 days, and the capacity of transplanted tooth germ to form hard tissue was explored histologically at postoperative one and two weeks. Moreover staining of sugar chains and compound polysaccharides were also compared with the unfrozen control group. Newly formed dent inogenesis was scatteringly observed at postoperative one week, and the dentin became thick at postoperative 2 weeks. Sugar chains as well as compound polysaccharides showed no definite deviation in expression and localization of pulp cells and odontoblasts between the experimental and control group. These above findings suggested that frozen tooth germs maintained dentinogenesis. Resorption of enamel or osseous ankylosis, both of which could occur in the trauma of tooth germ, was observed in those frozen tooth germs. The prevention of these changes is open to be solved hereafter The combination of tooth germ transplantation with the implantation of bone marrow tissue from the tibia formed slightly thicker dentin, although the residual bone marrow could not be confirmed after the implantation and such marrow tissue could be associated with the improved dentinogenesis, if any. Less