Invitromineral induction by phosphoprpteins-Change of PH in mineral induction

我们以前报道过，固定化磷酸蛋白和不溶性牙本质能够在亚稳态溶液中诱导矿物形成。然而，没有报道固定化磷蛋白诱导磷灰石形成的pH变化。在这项研究中，磷蛋白交联的琼脂糖珠，并在几个时间在亚稳溶液中测量pH值。我们研究了在矿物感应中使用电气工程评估的有用性。本研究中使用磷卵黄蛋白作为模型磷蛋白。将卵黄高磷蛋白固定于琼脂糖珠。使用了三种矿化溶液。在37 ℃下，在振荡器中，将10 μ L各样品置于3 ml亚稳态溶液中孵育。在几个时间点测量样品的pH并过滤。然后，通过原子吸收光谱法分析样品中的钙。在三种溶液中孵育开始后2.5h、3h、5h观察到卵黄高磷蛋白交联琼脂糖珠的pH下降。同时，钙吸收光谱法分析样品增多。结果表明，该方法简便、准确。

We previously reported that immobilized phosphoprpteins and insoluble dentin were able to induce to mineral formation in metastable solution. However, it were not reported pH change that immobilized phosphoprotein induce apatite formation. In this study, phosphoproteins were crosslinked to an agarose beads and measured pH at several time in metastable solution. We investigated that usefulness of evaluation using electrical engineering in mineral induction. Phosvitin was used as a model phosphoprotein in this study. Phosvitin was immobilized to agarose beads. Three mineralizing solutions were used. Ten microliters of each sample were incubated in 3ml of metastable solutions at 37℃ in a shaker. Samples were measured pH at several time points and filtered. Then, samples were analyzed for calcium by atomic absorption spectrometry. Phosvitin crosslinked agarose beads were observed fall of pH 2.5h, 3h, 5h after beginning of incubation in the three solution. At the same time, Samples increased in analysis for calcium absorption spectrometry. These results suggested this method was more simple and precision.