Nano area evaluation for adhesive mechanism of resin to dentin

本研究旨在探讨树脂对牙本质的粘接机理。采用纳米硬度计评价树脂-牙本质界面的力学性能，并对牙本质进行微拉伸粘结强度测试和用氩离子刻蚀技术对树脂-牙本质界面进行FE-SEM观察。本研究对市售自酸蚀系统进行了评价，使用ENT-1100 a纳米压痕仪（日本Elionix）测量了自酸蚀系统对树脂-牙本质界面粘结层的影响。从纳米硬度的结果来看，本研究中评估的市售自酸蚀粘合剂在粘合层硬度方面没有显着差异。两步自酸蚀体系的微拉伸粘结强度高于一步自酸蚀体系和一瓶自酸蚀体系。我们没能找到关于nanoh ...更多信息 采用氩离子束刻蚀的场发射扫描电镜（FE-SEM）观察了自酸蚀粘接剂与牙本质的超微结构相互作用。氩离子束刻蚀技术已被广泛用于树脂-牙本质界面的扫描电镜观察，描述了混合层由网状结构组成。用场发射扫描电镜观察了氩离子刻蚀后的网状结构，并观察了自酸蚀粘接剂的树脂-牙本质界面。结果表明，氩离子刻蚀树脂-牙本质界面的网状结构是由聚合的底漆和粘结树脂、混合层和部分暴露的胶原纤维组成，氩离子刻蚀30秒至2步自酸蚀粘结剂显示了较宽的混合层网状结构（2至5 μm）与先前报道的相似，而一瓶自粘合剂显示出窄的或没有网状宽度结果表明，牙本质与粘接剂之间的粘附力并不取决于混合层的宽度，牙本质的粘附力并不只是牙本质与粘接剂之间的机械咬合。少

The purpose of this study was to solve the adhesive mechanism of resin to dentin. The mechanical property of resin-dentin interface was evaluated by nanohardhess mesurements, and microtensile bond strength test to human dentin and FE-SEM observations of resin-dentin interface with argon ion etch technique were also performed. The self-etch systems which available on the market were evaluated in this study.We measured adhesive layer of resin-dentin interface by self-etch systems using an ENT-1100a Nanoindentation tester (Elionix, Japan). From the nanohardness results, commercially available self-etch adhesives evaluated in this study showed no significant difference in, hardness of adhesive layer.The human dentin bond strength of an available self-etch systems were evaluated by microtensile bond strength tests. The microtensile bond strength of 2-step self-etch systems were higher than 1-step or one bottled self-etch systems. We were not able to find about the relationship between nanoh … More ardness and bond strength of self-etch systems.The ultra-morphological interaction to human dentin of self-etch adhesive was observed by field-emission SEM (FE-SEM) with argon-ion (Ar-ion) beam etch technique. Ar-ion beam etch technique have been widely employed as SEM observation of resin-dentin interface, describing hybrid layer as composed of a meshwork structure. We examine the meshwork structure after ar-ion etch by FE-SEM, and also observed resin-dentin interface of self-etch adhesives. Our data suggested the meshwork strucrure on resin-dentin interface by ar-ion etch is composed of porymerized primer and bonding resin, hybrid layer and partially exposed collagen fibers.Ar-ion etching of 30-sec's to 2 step self-etch adhesives demonstrated a wide hybrid layer meshwork width (2 to 5 μm) similar to that previously reported, while one-bottled self adhesives showed narrow or no meshwork width (0 tO 0.5 μm) between dentin and adhesive after 30-sec's Ar-ion etching, It was suggested that width of hybrid layer is not necessary for dentin adhesion, and dentin adhesion is not formed only mechanical interlocking to dentin. Less