Study on the mechanical properties of hard tissues using nanoindentation tests

利用纳米压痕试验研究硬组织的力学性能

• 批准号: 15560064
• 负责人: SAKAMOTO Makoto
• 金额: $ 2.37万
• 依托单位: Niigata University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2003
• 资助国家: 日本
• 起止时间: 2003 至 2004
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-15560064/
• 关键词: Biomechanics; Strength of materials; Bone; Teeth; Nanohardness; Elastic modulus; Fracture strength; Mineral content

The mechanical properties of cortical bone are a consequence of the interaction between the mineral and collagen components. Small variations in the amount of mineral present in a section of bone can have a large influence on its mechanical properties. Studies have shown the correlations between Vickers microhardness and mineral content, and Vickers microhardness and elastic modulus using three-point and four-point bending tests. However, the precise nature of statistical relationship has yet to be established. Nanoindentation, which evolved from Vickers microhardness test, allows for measurement of mechanical properties of cortical bone at the nanometer scale. The nanoindentation technique has been applied to estimate mechanical properties of bone at a microscopic length scale, overcoming the limitations of traditional mechanical tests for small specimens. In this study, mechanical properties of bovine and porcine femoral cortical bone were measured by nanoindentation. For bovine plexiform and Haversian bone specimens, the averages for the longitudinal elastic moduli were 17.3±5.7 GPa and 10.6±5.1 GPa, respectively. The average elastic modulus measured for porcine plexiform bone was 13.7±4.5 GPa, and the average for porcine Haversian bone was 8.2±3.3 GPa. The hardness showed a tendency similar to the elastic modulus among all types of bone tissues. Next, the relationships between mechanical properties measured by nanoindentation and volumetric Bone Mineral Density(vBMD) measured by peripheral Quantitative Computed Tomography(pQCT) in femoral cortical bone from a variety of ages of rats were investigated. The specimen of rat cortical bone used here was Haversian bone. Experimentally measured elastic modulus correlated with vBMD(r=0.62〜0.76). It was found that a good correlation also existed between hardness and vBMD(r=0.85〜0.90) of bone tissues.

皮质骨的机械性能是矿物质和胶原成分之间相互作用的结果。骨切片中存在的矿物质量的微小变化可能对其机械性能产生很大影响。研究表明，维氏显微硬度和矿物含量之间的相关性，维氏显微硬度和弹性模量使用三点和四点弯曲试验。然而，统计关系的确切性质尚未确定。纳米压痕技术是由维氏显微硬度测试发展而来的，可以在纳米尺度上测量皮质骨的力学性能。纳米压痕技术已被应用于估计在微观长度尺度的骨的力学性能，克服了传统的小样本的力学测试的局限性。在这项研究中，力学性能的牛和猪股骨皮质骨纳米压痕测量。对于牛丛状骨和哈佛氏骨标本，纵向弹性模量的平均值分别为17.3±5.7 GPa和10.6±5.1 GPa。测量的猪丛状骨的平均弹性模量为13.7±4.5 GPa，猪哈弗氏骨的平均弹性模量为8.2±3.3 GPa。各种骨组织的硬度与弹性模量的变化趋势相似。接下来，研究了来自不同年龄大鼠的股骨皮质骨中通过纳米压痕测量的机械性能与通过外周定量计算机断层扫描（pQCT）测量的体积骨矿物质密度（vBMD）之间的关系。本文所用的大鼠皮质骨标本为哈佛氏骨。实验测得的弹性模量与vBMD相关（r=0.62〜0.76）。骨组织的vBMD与硬度也有良好的相关性（r=0.85 ~ 0.90）。

项目成果

Measurement of Mechanical Properties of Cortical Bone Using Nanoindentation Tests

使用纳米压痕测试测量皮质骨的机械性能

• 发表时间: 2003
• 期刊: Journal of Japanese Society for Clinical Biomechanics Vol.24
• 作者: 坂本信;坂本信;Makoto Sakamoto;Makoto Sakamoto;坂本信;坂本信;坂本信;坂本信;佐藤憲二;Makoto Sakamoto;Makoto Sakamoto;Makoto Sakamoto;坂本信;坂本信;坂本信;坂本信;坂本信;坂本信;坂本信;Makoto Sakamoto;Makoto Sakamoto
• 通讯作者: Makoto Sakamoto

坂本信: "生体硬組織の力学的特性"日本顎変形症学会総会. 13. 83-84 (2003)

Makoto Sakamoto：“生物硬组织的机械特性”日本颌畸形协会全体会议 13. 83-84 (2003)。

Nanoindentation Analysis of Mechanical Properties of Cortical Bone

皮质骨力学性能的纳米压痕分析

• 发表时间: 2004
• 期刊: The First Asian Pacific Conference on Biomechanics 04-203
• 作者: 坂本信;坂本信;Makoto Sakamoto;Makoto Sakamoto;坂本信;坂本信;坂本信
• 通讯作者: 坂本信

超微小押込み試験による皮質骨の力学的特性評価

超微压痕试验评价皮质骨力学性能

• 发表时间: 2003
• 期刊: 日本臨床バイオメカニクス学会誌 24
• 作者: 坂本信;坂本信;Makoto Sakamoto;Makoto Sakamoto;坂本信;坂本信;坂本信;坂本信;佐藤憲二;Makoto Sakamoto;Makoto Sakamoto;Makoto Sakamoto;坂本信;坂本信;坂本信;坂本信;坂本信
• 通讯作者: 坂本信

坂本信: "Elastic Modulus and Hardness of Cortical Bone Determined by Nanoindentation"13th International Conference on Mechanics in Medicine and Biology. 13. 162-163 (2003)

Shin Sakamoto：“通过纳米压痕测定皮质骨的弹性模量和硬度”第十三届国际医学和生物学力学会议。13. 162-163 (2003)。