Development of new prevention method for stomatitis caused by Candida induced by the use of denture in elderlies

为了研究白念珠菌病疫苗的可能性，我们每周将灭活的白色念珠菌涂抹在兔的腭扁桃体上，连续六周，从而诱导出针对白色念珠菌的唾液抗体。通过直接凝集和酶联免疫吸附试验，在唾液和血浆中发现了显著水平的白念珠菌抗体。免疫后唾液对白色念珠菌与克隆人上皮细胞的黏附有明显的抑制作用，而免疫前唾液对黏附无影响。从ELISA反应的选择性抑制和市售白念珠菌抗体的凝集结果显示，约80%的唾液抗体与甘露糖蛋白抗原之一-因子6发生反应，该抗原已被证明与白念珠菌与上皮细胞的黏附有关。因此，扁桃体内应用白色念珠菌可诱导唾液抗体，防止白色念珠菌附着于上皮细胞，因此在预防口腔念珠菌病方面应该是有用的。

To examine the possibility of a vaccine for candidiasis, we induced salivary antibodies to Candida albicans by weekly-applications of killed-C.albicans onto palatine tonsils of rabbits for six weeks. Significant levels of antibodies to C.albicans were seen in saliva and blood plasma by direct agglutination and ELISA. The saliva after immunization greatly inhibited C.albicans adherence to cloned human epithelial cells, while the saliva before immunization had no effect on the adherence. From the results of selective inhibition in ELISA reaction and agglutination by commercial antibodies to C.albicans, around 80% of the salivary antibodies were shown to react with one of the mannoprotein antigens, called factor 6 which has been shown to be involved of C.albicans adherence to epithelial cells. Therefore, tonsillar applications of C.albicans induce salivary antibodies that prevent C.albicans adherence to epithelial cells, and thus should prove useful in prevention of oral candidiasis.