Dissection of CrRLK1L signalling pathways regulating disease susceptibility and resistance to powdery mildew

长春花类受体样蛋白(CrRLK1L)是控制多种生理反应的重要受体。CrRLK1L费洛尼亚(FER)与其内源快速碱化因子(RALF)多肽配体一起，在植物生长、发育和免疫中发挥重要作用。FER是模式触发免疫(PTI)和抗菌素耐药的正向调节因子，通过促进配体诱导的微生物相关分子模式(MAMP)识别复合体的形成而促进Ralf多肽依赖的方式。相比之下，FER在白粉病侵染过程中起着敏感因子的作用，其在不同植物微生物相互作用中的作用机制尚不清楚。我们表明，其他已知的FER信号成分调节植物白粉病的适应，表明Ralf-FER代表了一条被适应的白粉菌劫持的途径。在这里，我们将分析FER在白粉病抗性过程中的机制基础，并剖析其在抗菌素抗性过程中的信号通路，以区分其功能多样性。该基因是一个与FER相关的CrRLK1L，但在白粉病的基础抗性中起着积极的作用，这表明CrRLK1L决定了阳性和阴性的感染结果。我们将探索拟南芥CrRLK1L的功能多样性，以阐明导致疾病易感性或抗性的分子机制。FER和相关的CRRLK1L在所有陆地植物中都是保守的，可能在不同物种之间具有相似的功能。大麦有六个相近的FER同源物，这表明该蛋白具有多样性，并提出了一个假设，即在不同的植物微生物相互作用中，单个大麦同源物接管了多功能拟南芥蛋白的特定角色。我们希望探索大麦中FER的功能多样性，为未来的作物改良策略提供可转移的知识。

CATHARANTHUS ROSEUS RECEPTOR-LIKE KINASE 1-LIKE (CrRLK1L) proteins are important receptors controlling diverse physiological responses. Together with its endogenous RAPID ALKALINISATION FACTOR (RALF) peptide ligands, the CrRLK1L FERONIA (FER) plays central roles during plant growth, development and immunity. FER is a positive regulator of pattern-triggered immunity (PTI) and antibacterial resistance by facilitating the formation of ligand-induced microbe-associated molecular pattern (MAMP) recognition complexes in a RALF peptide-dependent manner. By contrast, FER functions as a susceptibility factor during powdery mildew infection and the underlying mechanism for FER`s role during diverse plant microbe interactions remains unknown. We show that other known FER signalling components modulate plant powdery mildew accommodation, suggesting that RALF-FER represents a pathway hijacked by adapted powdery mildew fungi. Here, we will analyse the mechanistic basis of FER during powdery mildew susceptibility and dissect signalling pathways distinguishing its functional diversity during antibacterial resistance. THESEUS1 (THE1) is a CrRLK1L related to FER but plays a positive role in basal resistance to powdery mildew, suggesting that the repertoire of CrRLK1Ls determine positive and negative infection outcomes. We will explore the functional diversity of Arabidopsis CrRLK1Ls to elucidate the molecular mechanisms that result in disease susceptibility or resistance. FER and related CrRLK1Ls are conserved among all land plants with likely similar functions across species. Barley has six close FER homologs, suggesting that the protein diversified and raising the hypothesis that individual barley homologs take over specific roles of the multifunctional Arabidopsis protein during distinct plant microbe interactions. We want to explore the functional diversity of FER in barley to generate transferable knowledge for future crop improvement strategies.