Function of stromal cell and follicular dendritic cells on the functional differentiation and formation of lymph node

利用IL-2受体γ链敲除小鼠，研究其与基质细胞和滤泡树突状细胞的关系，认为基质细胞和树突状细胞影响淋巴结结构和微环境的调节。用电子显微镜观察了其超微结构。采用单克隆抗体检测细胞的特异性，即MOMA-1、MOMA-2、TCR-β链、CD45R、MIDC-8、FDC-M1、FDC-M2进行免疫组化。IL-2 Rγ链不导致TcRβ(+) T细胞数量减少，但导致淋巴结和脾脏中CD45R(+) B细胞数量减少。即使淋巴结结构不完整，也不影响位于T细胞区的MIDC-8阳性树突状细胞。单核细胞增多，脾脏中MOMA-1（+）细胞消失，淋巴结中MOMA-1（+）细胞增多。在该小鼠的一组基质细胞中发现了FDCs的前体。由于抗原刺激后，淋巴细胞间出现典型的FDC细胞质伸长过程。B细胞在未成熟FDC向成熟FDC的分化过程中未起关键作用。将IL-2R小鼠间质细胞分别培养为记忆T细胞、记忆B细胞和免疫复合物。当我们使用从T细胞区和B细胞区难以分离的未成熟淋巴结中分离FDC的方法时，我们可以得到前体细胞，结果表明IL-2受体γ链可能在FDC的分化过程中不起关键作用。该模型对间质细胞的功能构建、微环境和FDC的研究具有重要意义。

The relationship with stromal cells and follicular dendritic cells, which are considered to influence the structure of lymph node and the adjustment of microenvironment, was investigated used by IL-2 receptorγ chain knockout mouse.We had observed the ultrastructure by the electron microscopy. And the peculiar characteristic for the cell was detected by using monoclonal antibody, that is MOMA-1,MOMA-2,TCR-β chain,CD45R,MIDC-8,FDC-M1 and FDC-M2, for immuno histochemistry.1.The IL-2 Rγ chain did not lead the decrease the numbers of TcRβ(+) T cells, but decrease CD45R(+) B cells in the lymph node and spleen. It dose not influence the MIDC-8 positive dendritic cells which were located in the T cell area even if the structure of lymph node is not complete. The numbers of monocyte were increased, but MOMA-1(+) cells in spleen were disappeared, but much ones in lymph node. Precursor of FDCs was included in a group of stromal cells in this mouse. Because after the stimulation by the antigen, there was observed typical FDC elongated cytoplasmic processes with antigens between lymphocytes. The B cell did not play critical role to differentiate from immature FDC to mature.2.The stromal cells separated from IL-2R KO mouse were cultured with memory T cell, memory B cell and immune complexes. When we used the method of the separation FDC from immature lymph node which is difficult to separate from T cell areas and B cell area, we could get the precursor cellsOur result suggested that IL-2 receptorγ chain may do not play critical roles on the differentiation of FDC. This model is very useful for investigation of functional construction and microenvironment of stromal cells and FDC.