Effects of exercise on food intake and body mass in the Zucker fatty rat.

为了研究运动对Zucker脂肪大鼠食物摄入和体重的影响，我们计划进行这项研究。但由于O_2、CO_2分析仪和深度冷冻室的问题，不能得到满意的结果。低温驯化对肥胖大鼠体重增加的抑制作用更为明显。食物摄取量明显增加，但暴露在寒冷环境中的增加幅度远小于瘦鼠。棕色脂肪量在冷暴露期间显著增加，在脱暖后基本保持不变。肥胖大鼠的血糖、血脂、胰岛素、皮质酮、睾酮和瘦素较高，而甲状腺素较低。在冷驯化过程中，肥胖大鼠血浆甘油三酯和胰岛素显著降低。在Zucker肥胖大鼠中，暴露在寒冷环境下，由于热调节性产热作用增强，碳水化合物和脂质代谢改善，对体重、血浆甘油三酯和胰岛素的降低有良好的作用。长期暴露在寒冷中会增加非寒战产热，以维持体温。能量代谢发生显著变化。其结果是增加摄取量，抑制体重的增加和棕色脂肪组织（BAT）的质量，并发生形态变化。肝脏在能量代谢的调节中起着中心作用。在冷适应过程中，肝脏的形态也发生了变化。为了分析Zucker脂肪大鼠在冷驯化过程中适应性变化的机制，我们分析了冷驯化诱导的肝脏蛋白和BAT，并与瘦大鼠进行了比较。在冷驯化过程中，BAT中大约有60种不同的蛋白发生了变化。它们被分为与能量代谢相关的蛋白质、应激蛋白质和其他蛋白质。在冷适应过程中，肝脏中一些与碳水化合物代谢相关的蛋白质发生了变化。这些组织中蛋白的表达在Zucker脂肪大鼠和瘦大鼠中有所不同。少

To investigate the effects of exercise on food intake and body mass in the Zucker fatty rat, we planned this research. But we could not get adequate results because of the trouble of O_2 and CO_2 analyzer and deep freezer.Suppressive effect on body mass increment during cold acclimation was more profound in obese rats. Food intake was markedly greater, but the increase during cold exposure was much smaller than in lean rats. The brown fat mass increased significantly during cold exposure and kept about the same mass after deacclimation. Serum glucose, lipids, insulin, corticosterone, testosterone and leptin were higher, while thyroxine was lower in obese rats. During cold acclimation, plasma triglycerides and insulin was significantly lowered in obese rats. In Zucker obese rats, exposure to cold resulted in good effect on reduction of body mass, plasma triglycerides and insulin due to improvement of carbohydrate and lipid metabolism, induced by enhanced thermoregulatory thermogenesis.L … More ong-term cold exposure causes increase in non-shivering thermogenesis to maintain body temperature. The energy metabolism changes remarkably. It results in increase of food intake, inhibition of increase in body weight and in mass of brown adipose tissue(BAT) with morphological changes. Liver plays a central role in regulation of energy metabolism. Changes in Morphological appearance of liver were also observed during cold adaptation. To analyze the mechanism of adaptive changes in Zucker fatty rats during cold acclimation, proteins of liver and BAT induced by cold acclimation were analyzed and they were compared with those of lean rats.During cold acclimation, approximately 60 different proteins in BAT were changed. They were classified into proteins related to energy metabolism, stress proteins and others. Some proteins related to carbohydrate metabolism were changed in the liver during cold adaptation. Expressions of proteins in these tissues were somewhat different in Zucker fatty rats and lean rats. Less