ION TRANSPORT,LIPID METABOLISM AND HEAT PRODUCTION DURING COLD ACCLIMATION IN RAT BROWN ADIPOSE TISSUE CELLS.

研究了暖、冷（8w）驯化和再驯化（冷，4w：暖，4w）对棕色脂肪组织细胞内离子转运、脂质代谢和产热的影响。用电子探针x射线显微分析和膜片钳技术对细胞器进行了研究。低温驯化大鼠细胞的高压电镜显示线粒体肿胀，组织细胞脂滴减少和/或变形。冷驯化细胞线粒体的x射线微分析显示，与热驯化细胞相比，冷驯化细胞的K和Cl浓度较低，Ca浓度较高。再驯化大鼠的钙含量下降到温驯化大鼠的水平。利用膜片钳对重组膜细胞器进行了冷驯化过程中电压和H+敏感电流的测量。实验结果表明，冷驯化过程中，细胞细胞器内膜存在一个较小的电导和一个相对较大的电导，分别对应于cl通道和Ca2+依赖性K+通道。

The effects of warm or cold (8w) acclimation and reacclimation (cold, 4w : warm, 4w) on the intracellular ion transport, lipid metabolism and heat production of the brown adipose tissue cells were studied. The cells were investigated by electron probe X-ray microanalysis and organelles by the patch clamp technique.The high voltage electron micrographs of the cells of cold acclimated rats revealed mitochondrial swelling and a decrease and/or deformation of lipid droplets of tissue cells. X-ray microanalysis of the mitochondria of the cold acclimated cells showed lower K and Cl, and higher Ca concentrations than those of the warm acclimated ones. The Ca of the reacclimated rat decreased to the level of the warm acclimated rat. The reconstituted membrane of the organelle was investigated by patch clamp to measure the voltage and-or H+ sensitive currents during cold acclimation. The experiments revealed the presence of a small and a relatively large conductance that correspond to the Cl-channel and the Ca2+dependent K+channel in the inner membrane of the organelle of the cells during cold acclimation.