ION TRANSPORT,LIPID METABOLISM AND HEAT PRODUCTION DURING COLD ACCLIMATION IN RAT BROWN ADIPOSE TISSUE CELLS.

大鼠棕色脂肪组织细胞冷驯化过程中的离子传输、脂质代谢和产热。

• 批准号: 07670095
• 负责人: HORI Seiki
• 金额: $ 1.34万
• 依托单位: HYOGO COLLEGE OF MEDICINE
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670095/
• 关键词: BROWN ADIPOCYTE; HEAT PRODUCTION; INTRACELLULAR ION CONTENT; COLD ACCLIMATION; THERMOGENIN; LIPID METABOLISM

The effects of warm or cold (8w) acclimation and reacclimation (cold, 4w : warm, 4w) on the intracellular ion transport, lipid metabolism and heat production of the brown adipose tissue cells were studied. The cells were investigated by electron probe X-ray microanalysis and organelles by the patch clamp technique.The high voltage electron micrographs of the cells of cold acclimated rats revealed mitochondrial swelling and a decrease and/or deformation of lipid droplets of tissue cells. X-ray microanalysis of the mitochondria of the cold acclimated cells showed lower K and Cl, and higher Ca concentrations than those of the warm acclimated ones. The Ca of the reacclimated rat decreased to the level of the warm acclimated rat. The reconstituted membrane of the organelle was investigated by patch clamp to measure the voltage and-or H+ sensitive currents during cold acclimation. The experiments revealed the presence of a small and a relatively large conductance that correspond to the Cl-channel and the Ca2+dependent K+channel in the inner membrane of the organelle of the cells during cold acclimation.

研究了热或冷（8周）驯化和再驯化（冷，4周：温，4周）对棕色脂肪组织细胞的细胞内离子转运、脂质代谢和产热的影响。通过电子探针X射线微量分析和膜片钳技术对细胞器进行研究。冷适应大鼠细胞的高压电子显微照片显示线粒体肿胀和组织细胞脂滴减少和/或变形。对冷驯化细胞线粒体的 X 射线微量分析显示，与热驯化细胞相比，K 和 Cl 较低，Ca 浓度较高。重新适应的大鼠的 Ca 降低至热适应大鼠的水平。通过膜片钳研究细胞器的重构膜，以测量冷驯化期间的电压和/或H+敏感电流。实验揭示了冷驯化过程中细胞细胞器内膜中存在与 Cl 通道和 Ca2+ 依赖性 K+ 通道相对应的较小和相对较大的电导。

项目成果

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Sasaki,S.,Nakagaki,I.,Hori,K.,Tsujita,J.,Hori,S .,Yahata,T.,Kuroshima,A: "Intracellular ion transport in mitochondrial membrance during cold acclimation in rat brown adipose tissue." Ed. Nagasaka,T. & Milton,A.S.In : Body temperature and melabolism,. IPEC

Sasaki，S.，Nakagaki，I.，Hori，K.，Tsujita，J.，Hori，S .，Yahata，T.，Kuroshima，A：“大鼠棕色脂肪组织冷适应过程中线粒体膜的细胞内离子转运。

Sasaki, S., Nakagaki, I., Hori, K., Tsujita, J., Hori, S., Yahata, T., Kuroshima, A.: "Intracellular ion transport in mitochondrial membrane during cold acclimation in rat brown adipose tissue." Ed.Nagasaka, T.& Milton, A.S.In : Body temperature and metab

Sasaki，S.，Nakagaki，I.，Hori，K.，Tsujita，J.，Hori，S.，Yahata，T.，Kuroshima，A.：“大鼠棕色脂肪组织冷适应过程中线粒体膜的细胞内离子转运