A study of the pigment which inhibits enzymatic reaction

当一些色素渗透到法医或人类学样本中时，如头发和人骨，众所周知，在许多情况下，DNA分型是不可能的，因为PCR反应没有进展。我进行这项研究的目的是建立从含有色素的样本中进行DNA分型的方法。首先，我试图去除抑制PCR反应的染发剂的色素，但不幸的是找不到有效的方法来做到这一点。然而，即使在用永久染发剂处理的头发的情况下，很明显，通过使用这种方法提取的模板DNA，聚合酶链式反应是成功的，而不会溶解Pilar皮质。另一方面，我注意到了埋葬土壤中的天然色素，它抑制了聚合酶链式反应。然后，我尝试从古骨中提取DNA，而不受这些色素成分的酶抑制。因此，即使在从染色的骨骼中提取的DNA中，我独创的用硅粉提取DNA的方法也可以提高DNA分型的成功率。在这项研究中，我成功地用天然和合成色素克服了大部分酶的抑制。

When some pigment infiltrate into a forensic or an anthropological sample such as a hair and a human bone, it is known that DNA typing is impossible in many cases because PCR reaction does not advance. I carried out this study for the purpose of establishing methods for DNA typing from the sample in which the pigment was included.At first, I tried to remove the pigment of hair dye which inhibits PCR reaction, but could not unfortunately find effective methods to do it. However, even in the case of a hair treated with perpetual hair dye, it became clear that PCR is successful by using template DNAs extracted by the method without the dissolution of the pilar cortex. On the other hand, I paid my attention to natural pigment, which inhibited PCR, in the burial soil. Then, I tried to extract DNA from ancient bone without enzyme inhibition by these pigment ingredients. As a result, even in DNA extracted from the dyed bones, a success rate of DNA typing could be raised by my original method of DNA extraction using silica powder.In this study, I succeeded in overcoming most, of the enzyme inhibition with natural and synthetic pigments.