Development of improving method for respiratory function in newborn diaphragma hernia

虽然已有报道通过5-氮杂胞苷的刺激将骨髓细胞导入心肌细胞，但其效率较低，仍有许多问题需要解决。研究了利用外周血单核细胞进行心肌再生的新方法。方法：用单核细胞分数采集器采集外周血单核细胞。该方法获得的单核细胞总数为1.8×10~(-9)细胞。CD14阳性细胞计数为0.06×10~(-7)。对7例疼痛的ASO患者的肢体注射富单核细胞组分。结果：注射富单核细胞组分后API由0.32提高到0.49。所有患者使用止痛药的需求均显著减少。4例患者停用五唑西林。我们认为这一效应是由于缺血肢体中单核细胞新生血管所致。这种新生血管的机制将成为心肌梗死后心肌再生的新方法。我们认为获取单核细胞比例的方法非常简单，因此我们可以在心脏手术的手术室中使用这种方法。

Although it has been reported that bone marrow cell could be introduced to cardiomyocyte by the stimulation of 5-azacytidine, its efficacy has been low and many points must be resolved were remained. We studied the new method for myocardial regeneration using peripheral monocyte.Method : Peripheral monocyte were collected by using monocyte fraction collector from peripheral blood. Total ammount of monocyte derived from this method was 1.8x10^9 cells. CD14 positive cells count was 0.06x10^7. Moncyte rich fraction was injected to the lower limb of painful ishemic 7 patients suffering ASO.Results : API was improved from 0.32 to 0.49 after injection of monocyte rich fraction. The need of use of anti-pain drug were dramacally reduced in all patients. 4 patients stopped the use of pentazocin. We consider that this effect is produced by the neovascularization by monocytes in the ischemic limb. This mechanism of neovascularization will be the new method for myocardial regeneration in the oschemin myocardium after infarction. We think that the method for getting monocyte fraction is very easy, so that we can use this method in the operating room for cardiac surgery.