The role of bone cytokine, CBFa-1, OPG and ODF in the mechanism of bone metastases with prostate cancers

前列腺癌成骨转移的机制尚不清楚，但可能与成骨细胞和破骨细胞的平衡有关。成骨细胞和破骨细胞的调节因子(骨细胞因子、OPG、ODF和CBFA-1)在骨转移中起重要作用。我们评估了骨调节因子基因在癌细胞中的定量表达。我们使用激光捕获显微切割技术仅从前列腺癌、膀胱癌和肾癌标本中去除癌细胞。实时定量RT-PCR检测BMP-2、BMP-6、CBFA-1、PHT-RP、ODF和OPG的表达。以各因子/β-肌动蛋白×1000m RNA的相对值表示。前列腺癌组织中BMP-2、BMP-6、OPG、CBFA-1、PTH-RP和ODF值分别为14.33±3.30，21.18±1.35，4.71±1.29，3.47±1.62，24.45±8.10和1.2 9±0.6 5。BMP2、BMP6、OPG、CBFA1、PTHRP和ODF值在肾癌中分别为0.95±0.20，1.30±0.60，1.52±0.36，0.55±0.36，0.29±0.13和2.2 0±1.2 8。BMP-2、BMP-6、OPG、CBFA-1、PTH-RP和ODFmRNA在膀胱癌组织中的表达分别为1.19±0.56，0.18±0.11，0.26±0.14，0.62±0.20，0.12±0.06和0.13±0.09.前列腺癌的OCIF、PTH-RP和BMP-2、BMP-6的相对值明显高于膀胱癌和肾癌。前列腺癌患者BMP-2、BMP-6和PTH-RP的相对值明显高于其他因素。骨形成调节因子可能在前列腺癌骨转移机制中起重要作用。阐明骨转移的机制将有助于晚期前列腺癌的治疗。

The mechanism for the osteoplastic metastases in prostate cancer is not clearly known but osteoplastic metastases might be correlated with the balance of osteoblast and osteoclast cell. The regulator factors of osteoblast and osteoclast cells (bone cytokine, OPG, ODF and CBFa-1) mignt have an important role in bone metastases. We evaluated the quantifcation of gene expression of bone regulator factors in the cancer cells. We removed only the cancer cells from the specimens of prostate cancer, bladder cancer and renal cell cancer using laser-captured microdissection. We measured the real-time RT-PCR quantification of BMP-2, BMP-6, CBFa-1, PHT-rP, ODF and OPG. The relative values of mRNA were presented as each factors/β-actin ×1000. The relative values of BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF ware 14.33±3.30,21.18±1.35,4.71±1.29,3.47±1.62,24.45±8.10 and 1.29±0.65 in prostate cancer, respectively. The BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF were 0.95±0.20,1.30±0.60,1.52±0.36,0.55±0.36,0.29±0.13 and 2.20±1.28 in renal cell cancer, respectively. The mRNA expression of BMP-2, BMP-6, OPG, CBFa-1, PTH-rP and ODF were 1.19±0.56,0.18±0.11,0.26±0.14,0.62±0.20,0.12±0.06 and 0.13±0.09 in bladder cancer, respectively. The relative OCIF, PTH-rP and BMP-2, BMP-6 values in prostate cancer were significantly higher than that in bladder cancer and renal cell cancer. The relative BMP-2, BMP-6 and PTH-rP values were significantly higher than other factors in prostate cancer. The regulator factors of bone formation might be an essential role in mechanism of bone metastases from prostate cancer. Elucidating the mechanism of bone metastasis will be useful to treat the advanced prostate cancer.