Changes of Serum Lipid Levels induced by Periodontal Infection in Rats

近年来，有证据表明牙周病患者血清中抗银杏卟啉单胞菌抗体效价、胆固醇、甘油三酯和血糖水平均显著高于健康人。提示牙周病可影响血脂和血糖的代谢。为探讨实验性牙周炎对大鼠血清甘油三酯、血糖、细胞因子α和C反应蛋白水平的影响，将29只雌性Wistar大鼠分为PG感染组(n=9)、PG非感染组(n=8)和对照组(n=12)。在PG感染组和未感染组中，通过在下颌第一磨牙(M1)周围施加一个小的弹性环来诱导实验性牙周炎。1周后，将PG浸泡的丝线或无菌丝线插入牙周袋，并用弹性环固定在PG感染组和未感染组大鼠的M1周围，Re…更具体地说。术后2周更换真丝缝线和弹力环。在更换缝线和弹力环两周后，从心脏抽血并摘除下颌骨，处死大鼠。对照组大鼠不治疗并维持5周，PG感染组(2.20±0.14 mm)和未感染组(2.14±0.13 mm)与对照组(1.36±0.11 mm)相比，牙槽骨明显丢失。而PG感染组与非感染组之间差异无统计学意义。PG感染组血糖水平(143.1±6.1 mg/dl)显著高于PG非感染组(132.3±4.8 mg/dl)和对照组(132.7±9.4 mg/dl)。在甘油三酯方面，由于个体差异很大，三组之间没有显著差异。各组肿瘤坏死因子-α水平均低于可检测水平(0.7pg/ml)。C反应蛋白水平无明显差异，认为牙周组织PG感染可引起血糖升高。然而，被认为与血糖水平升高有关的肿瘤坏死因子-α在外周血中没有检测到。需要进一步的研究来确定牙周组织的Pg感染和血糖水平升高之间的潜在联系的机制。较少

In recent years, some evidence has been presented that serum antibody titer against Porphyromonas gingzvalis(Pg), cholesterol, triglyceride and blood glucose levels were significantly higher in periodontally diseased patients than in healthy subjects. Thus, it is suggested that periodontal disease could influence the metabolism of lipids and glucose. This study examined the effects of experimentally induced periodontitis on the serum level of triglyceride, glucose, cytokine(TNF-α) and C-reactive protein(CRP) in rats.Twenty-nine female Wistar rats were divided into the following 3 groups : Pg-infected group(n=9), Pg-non infected group(n=8) and control group(n=12). Experimental periodontitis was induced by applying a small elastic ring around the mandibular first molar(M1) in Pg-infected and non infected groups. After one week, Pg soaked or sterile silk sutures were inserted into periodontal pockets and fixed by elastic rings around M1 of rats in the Pg-infected or non infected group, re … More spectively. Silk sutures and elastic rings were changed after 2 weeks. Two more weeks after changing the sutures and elastic rings, rats were sacrificed by drawing blood from the hearts and removed mandibular bones. Rats of control group were not treated and maintained for 5 weeks.Significant alveolar bone loss was found in the Pg-infected (2.20±0.14mm) and non infected (2.14±0.13mm) groups compared with that in the control group (1.36±0.11mm). However, there was no significant difference between Pg-infected and non infected groups. The blood glucose level was significantly higher in the Pg-infected group(143.1±6.1mg/dl) than that in the Pg-non infected (132.3±4.8mg/dl) and control groups (132.7±9.4mg/dnl). With regard to triglyceride, there was no significant difference in the three groups because of large individual differences. TNF-α levels of all groups were below the detectable level(<0.7pg/ml). There was no significant difference in the CRP level.It was considered that Pg-infection of periodontium could raise the blood glucose level. However, TNF-α, which has been considered involved in the increased glucose level, was not detected in peripheral blood. Further studies are needed to determine the mechanisms underlying the association between Pginfection of the periodontium and an increase in the glucose level. Less