Accumulation of Bisphenol A in the body

双酚A是一种内分泌干扰物质，主要影响胎儿。研究其在胎鼠体内的分布、体内蓄积和体外排泄情况，将为该材料的安全性提供非常重要的信息。妊娠小鼠按4.3 mg BPA/kg体重(50uci/只)腹腔注射C-双酚A。分别于注射后1小时、1天、3天和5天处死小鼠。将处死的小鼠全身立即冰冻，用冰冻显微切割机沿小鼠身体中心矢状切割出约10个骨灰盒厚的全身切片。注射后第五天出生的五只小鼠也以同样的方式进行处理。用显像板和荧光/放射性同位素图像分析仪对小鼠的放射自显影图像进行分析，注射1h后，~(14)C-双酚A分布到包括胎儿在内的全身。在肝脏、回肠、颌下腺、肾、胆和子宫壁中检测到较高浓度的β-lt；14&gt；C。胎儿的肝脏已经比胎儿身体的其他部分表现出更多的浓缩。24小时后，除肝脏、横结肠和腹膜下的空肠外，其余组织中的β-14-C含量均呈下降趋势。3日龄时，肝、肾皮质、回肠、空肠和结肠为敏感器官，且以腹壁、肝脏、肾皮质、回肠、空肠和结肠为敏感器官。到第5天时，全身放射性同位素活性较弱，仅限于肝脏、肾皮质、肠和结肠。尽管其活性非常弱，但仍被传递给幼鼠，并在肝区检测到。

Bisphenol A is one of the endocrine disturbing materials, which gives its influence mostly to the fetus. It will give a very important information for the safety of the material to investigate its distribution to the whole body, accumulation in the body and excretion to outside of body when incorporated to the body in the time of fetus.Four mice in the thirteenth day of pregnancy were used. ^<14>C-bisphenl A was injected intraperitoneally to the pregnant mice at 4.3 mg BPA/kg body weight (50 uCi/mouse). Each mouse was killed at one hour, one day, three days and five days after the injection. The sacrificed entire mouse body was immediately frozen and approximately 10 urn-thick whole body section was cut out sagittaly along the center of the mouse body using cryomicrotome. Five mice delivered by the fifth day after the injection were processed in the same way. The auto radiographic image of the mice was obtained and analyzed using the imaging plate and fluorescent/radioisotope image analyzer.The ^<14>C-bisphenol A distributed to the whole body including the fetuses one hour after the injection. High concentration of ^<14>C was detected in liver, ileum, submandibular gland) kidney, gall bladder and wall of uterus. The liver in the fetus already showed more concentrated ^<14>C than the other part of fetus body. At 24 hours, the ^<14>C was decreasing in its amount in the whole body except in liver, transverse colon and also in the jejunum right under the peritoneal. At three days, ^<14>C was detected mostly in the abdominal cavity, especially the fetus wall) liver, cortex of kidney, ileum, jejunum and colon were sensitive organs. By fifth day, the radioisotope activity of the whole body was much weaker and the activity was limited to liver, cortex of kidney, intestine and colon. The ^<14>C activity was transmitted to baby mice and detected in the liver area even though its activity was very weak.