laser mouse heart ultrasound-photoacoustic imaging systems

马丁路德大学哈勒-威滕贝格分校医学系的心脏病学、心脏外科和肿瘤学专科工作组正在致力于需要现代高灵敏度超声成像和功能深度分析方法的项目。小动物高频超声心动图超声设备与光声激光模块相结合，必须以生理上的高搏动频率(每分钟400-600次)固有地共同记录来自小鼠心脏的信号和其他深部器官的高分辨率信号。为了对小鼠模型中的心脏/肿瘤和小血管(体重在15到45克之间)进行解剖和功能分析，高时间和空间分辨率以及实时成像(例如，用于评估心血管活性治疗)是必要的。心电触发的数字重建和重新连接来自不同心脏周期的图像预计将产生高达每秒10,000帧的图像密度。标准的超声成像方法以及心功能参数、彩色多普勒、脉搏波多普勒、能量多普勒、组织多普勒和组织向量分析等功能分析方法必须是可行的。结构容积法必须可用于确定器官、梗死大小和周缘扩大，以及这些物体随时间的三维测量。这里，必须通过呼吸和心电信号对信号进行归一化，以避免运动伪影。通过对比标记物，可以看到组织的血流灌注。通过将经典的超声设备与光声系统相结合，将进行分子分析，如组织充氧和灌流(通过检测氧合和脱氧血红蛋白)以及光活性物质或分子标记物的光谱测量。因此，(重新)血管化和致癌过程可以被量化。其他波长范围被用来检测造影剂，这些光声信号的光谱分离应该使使用几个标记成为可能，只有非放射性的。小鼠将在异氟醚麻醉系统中接受监测，以监测生理功能，如心电图，以便以后评估干预实验，可单独分析。为了避免调查人员的运动伪影，该设备应该可以通过语音控制进行操作。

Working groups of cardiological, cardio-surgical and oncological specialization at themedical faculty of the Martin-Luther-University Halle-Wittenberg are working onprojects that require modern, highly sensitive ultrasound imaging with functionallyin-depth analysis methods. The small animal high-frequency echocardiographicultrasound device-combination with a photoacoustic laser module must inherently co-registrate both signals from mouse hearts at their physiologically high beating rate (400-600 beats per minute) and other deep organs in high resolution. For the purpose of anatomical and functional analyses of hearts/neoplasiae and small vessels in the mouse model (weight between 15 and 45g), a high temporal and spatial resolution is necessary, as well as real-time imaging (e.g. for the evaluation of cardiovascular active therapeutics). An ECG-triggered digital reconstruction and reconnection of images from different cardiac cycles is expected to result in image densities of up to 10,000 frames per second. Standard echographic imaging methods, as well as functional analysis methods such as cardiac functional parameters, color Doppler, Pulse-wave Doppler, Power Doppler, Tissue Doppler, and tissue vector analyses must be feasible. Structural volumetry must be available to determine organs, infarct sizes and circumferential enlargements, as well as the three-dimensional measurement of these objects over time. Here, the signals must be normalized via the respiratory and ECG signal so that motion artifacts can be avoided. By means of contrast markers, tissue perfusions can be visualized.By combining the classical ultrasound device with a photoacoustic system, molecular analyses will be performed, such as tissue oxygenation and perfusion (via detection of oxy- and deoxyhemoglobin) and spectroscopic measurements of photoactive substances or molecular markers. Thus, (re)vascularizing and oncogenic processes can be quantified. Other wavelength ranges are used to detect contrast agents, and the spectral separation of these photoacoustic signals should make the use of several markers possible, exclusively non-radioactive. The mice will be monitored at an isoflurane anesthesia system to monitor the physiological functions such as ECG for later evaluation of intervention experiments, separately analyzable. To avoid movement artifacts by the investigator, the device should operable by voice control.