Integration of chromosome mand linkage maps of Lotus japonicus, Miyakogusa as a model plant of legume

宫古岛荷花（Lotus japonicus, Miyakojima mg - g）是豆科植物细胞学模式植物。我开发了定量粗线染色体图谱，并将它们与其他Lotus染色体图谱结合起来，整合它们的信息，以进行有效和准确的基因组分析。Pachytene染色体采用定量染色体图像分析和FISH方法，使用4个重复序列和28个转化能力人工染色体（TAC）克隆进行更高分辨率的定位。利用染色体图像分析系统（CHIAS）测定粗线染色体的凝聚模式（CP），确定了基因位点的地址。我们整合了三张地图；有丝分裂前期染色体图谱，减数分裂粗线染色体图谱和连锁图谱。整合图谱显示了TAC克隆在染色体上的精确物理位置，并与染色体高级结构相关，代表着着丝粒、端粒、细胞学特异性异染色质和常染色质。此外，我们比较了由重组频率得出的遗传距离与粗线染色体图谱上DNA标记之间的物理距离。

Lotus japonicus, Miyakojima MG-20 is a cytological model plant of the legume family. I have developed quantitative pachytene chromosome maps and combined them with the other Lotus chromosome maps to integrate their information for effective and accurate genome analysis. Pachytene chromosomes applied the quantitative chromosome image analysis and FISH methods using four repetitive sequences and 28 transformation-competent artificial chromosome (TAC) clones are used for higher resolution mapping. The addresses of gene loci had been identified by chromosome image analysis system (CHIAS), which measures the condensation pattern (CP) of the pachytene chromosome. We have integrated three maps ; mitotic prometaphase chromosome map, meiotic pachytene chromosome map and linkage map. The integrated maps demonstrated precise physical positions of TAC clones on chromosomes, and associated with chromosomal higher structures, representing in centromeres, telomeres, cytologically specific heterochromatin and euchromatin. Moreover, we compared genetic distances derived from recombination frequency with physical distances between DNA markers on pachytene chromosome maps.